Abstract

We are using the tRNA genes of the silkworm, Bombyx mori, to study transcriptional regulation of eukaryotic genes. We are analyzing the transcriptional properties of cloned genes in cell free extracts prepared from several Bombyx tissues and from Xenopus oocytes to investigate the factors required for correct transcription of these genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM025388-08
Application #
3272974
Study Section
Genetics Study Section (GEN)
Project Start
1978-08-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Oregon
Department
Type
Graduate Schools
DUNS #
948117312
City
Eugene
State
OR
Country
United States
Zip Code
97403

  Publications

Ouyang, C; Martinez, M J; Young, L S et al. (2000) TATA-Binding protein-TATA interaction is a key determinant of differential transcription of silkworm constitutive and silk gland-specific tRNA(Ala) genes. Mol Cell Biol 20:1329-43
Trivedi, A; Young, L S; Ouyang, C et al. (1999) A TATA element is required for tRNA promoter activity and confers TATA-binding protein responsiveness in Drosophila Schneider-2 cells. J Biol Chem 274:11369-75
Young, L S; Ahnert, N; Sprague, K U (1996) Silkworm TFIIIB binds both constitutive and silk gland-specific tRNA Ala promoters but protects only the constitutive promoter from DNase I cleavage. Mol Cell Biol 16:1256-66
Smith, T P; Young, L S; Bender, L B et al. (1995) Silkworm TFIIIA requires additional class III factors for commitment to transcription complex assembly on a 5S RNA gene. Nucleic Acids Res 23:1244-51
Dunstan, H M; Young, L S; Sprague, K U (1994) TFIIIR is an isoleucine tRNA. Mol Cell Biol 14:3588-95
Dunstan, H M; Young, L S; Sprague, K U (1994) tRNA(IleIAU) (TFIIIR) plays an indirect role in silkworm class III transcription in vitro and inhibits low-frequency DNA cleavage. Mol Cell Biol 14:3596-603
Palida, F A; Hale, C; Sprague, K U (1993) Transcription of a silkworm tRNA(cAla) gene is directed by two AT-rich upstream sequence elements. Nucleic Acids Res 21:5875-81
Dieci, G; Duimio, L; Coda-Zabetta, F et al. (1993) A novel RNA polymerase III transcription factor fraction that is not required for template commitment. J Biol Chem 268:11199-207
Young, L S; Dunstan, H M; Witte, P R et al. (1991) A class III transcription factor composed of RNA. Science 252:542-6
Young, L S; Rivier, D H; Sprague, K U (1991) Sequences far downstream from the classical tRNA promoter elements bind RNA polymerase III transcription factors. Mol Cell Biol 11:1382-92

Showing the most recent 10 out of 15 publications