The influence of solvent and sequence on peptide conformation is to be studied, using both cyclic peptides synthesized for the purpose and analogs of luteinizing hormone releasing hormone, LHRH. Sequences that produce conformationallly stable cyclic peptides will be sought for use in designing analogs of biologically active peptides. Solution conformations of the cyclic peptides will be compared with crystal structures where possible. Relationships between solution conformation and biological activity will be sought for the LHRH derivatives. Nuclear magnetic resonance will be the principal spectroscopic tool. Continued development of NMR methods for peptide conformation will be directed to methods for measuring solvent exposure of peptide backbone protons. Cyclic peptides will be used as models from which to obtain qualitative and quantitative measures of interactions between peptide and solvents.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026071-07
Application #
3273535
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1979-05-01
Project End
1987-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Illinois Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
City
Chicago
State
IL
Country
United States
Zip Code
60616
Kopple, K D; Wang, Y S (1989) N.m.r studies of internal mobility in a cyclic tetrapeptide. Int J Pept Protein Res 33:82-5
Romanowska, K; Kopple, K D (1987) Molecular mechanics investigation of the flexibility of some cyclic peptides. Int J Pept Protein Res 30:289-98
Vishwanath, C K; Go, A; Parameswaran, K N et al. (1986) Conformations of cyclic octapeptides. 4. Diastereomers of cyclo(Gly-Pro-Phe-Ala-Asn-Ala-Val-Ser). Int J Pept Protein Res 28:428-36
Timkovich, R; Vavra, M R (1985) Proton NMR spectroscopy of sulfmyoglobin. Biochemistry 24:5189-96
Timkovich, R; Cork, M S; Taylor, P V (1985) 1H NMR spectroscopy of cytochrome cd1 derivatives. Arch Biochem Biophys 240:689-97