The Complexity of Chromosomal Structure and Assembly

Weintraub, Harold M.

Abstract

The MyoD cDNA, when expressed under the control of a viral LTR and transfected into a variety of fibroblast and adipoblast cell lines, converts these cells to muscle. While not yet proven MyoD acts as if it were a master regulatory gene for myogenesis. The MyoD protein is nuclear and preliminary work suggests it binds DNA. The present proposal is to: (1) Characterize MyoD using detailed mutagenic analysis. (2) Study its binding to specific DNA sequences; study its interaction with other cellular proteins. (3) Explore preliminary indications that MyoD positively activates its own synthesis but negatively activates its mRNA utilization.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Research Project (R01)
• Project #: 5R01GM026176-12
• Application #: 3273674
• Study Section: Molecular Cytology Study Section (CTY)
• Project Start: 1979-05-01
• Project End: 1994-04-30
• Budget Start: 1990-05-01
• Budget End: 1991-04-30
• Support Year: 12
• Fiscal Year: 1990

Institution

• Name: Fred Hutchinson Cancer Research Center
• DUNS #: 075524595
• City: Seattle
• State: WA
• Country: United States
• Zip Code: 98109

Publications

Laue, T M; Starovasnik, M A; Weintraub, H et al. (1995) MyoD forms micelles which can dissociate to form heterodimers with E47: implications of micellization on function. Proc Natl Acad Sci U S A 92:11824-8

Cross, F R (1990) The DAF2-2 mutation, a dominant inhibitor of the STE4 step in the alpha-factor signaling pathway of Saccharomyces cerevisiae MAT alpha cells. Genetics 126:301-8

Cross, F R (1990) Cell cycle arrest caused by CLN gene deficiency in Saccharomyces cerevisiae resembles START-I arrest and is independent of the mating-pheromone signalling pathway. Mol Cell Biol 10:6482-90

Richardson, H E; Wittenberg, C; Cross, F et al. (1989) An essential G1 function for cyclin-like proteins in yeast. Cell 59:1127-33

Cross, F R (1989) Further characterization of a size control gene in Saccharomyces cerevisiae. J Cell Sci Suppl 12:117-27

Cross, F R (1988) DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae. Mol Cell Biol 8:4675-84

Bentley, D L; Groudine, M (1988) Sequence requirements for premature termination of transcription in the human c-myc gene. Cell 53:245-56

Hutchison, N (1987) Lampbrush chromosomes of the chicken, Gallus domesticus. J Cell Biol 105:1493-500

Hutchison, N; Weintraub, H (1985) Localization of DNAase I-sensitive sequences to specific regions of interphase nuclei. Cell 43:471-82