Abstract

High resolution mass spectrometry will be employed in studying biomedically important compounds, stressing the new technique of fast atom bombardment mass spectrometry (FABMS). A high field mass spectrometer will be modified to observe ions to m/z 10,000 at maximum accelerating potential (8KV). A variety of ion guns as well as postanalyzer acceleration will be employed to enhance sensitivity. Several matrices will be studied to enhance FAB ion production. Mass markers will be prepared to facilitate computerized data collection and accurate mass measurements of both positive and negative ions, and optimization of high resolution measurements will be studied in ion sources of different designs. Collisional activation will be employed to enhance FAB-induced fragmentation. A high performance liquid chromatograph will be coupled to a FAB mass spectrometer by a modified moving belt apparatus. Compounds to be studied by these techniques include involatile or thermally unstable antibiotics (especially peptides and polyenes), antiviral, antitumor and antimicrobial marine natural products, other peptides (enzymic digests of proteins, fluorogenic peptides, peptide derivatives of drugs and suicide inhibitors), polynucleotides, glycolipids, including gangliosides, coenzymes, terpene pyrophosphates, modified porphyrins, organometallic analogs of metalloenzymes, carcinogenic metabolites, organic compounds with hypervalent atoms, sulfonium salts, and metal carbonyls.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM027029-19
Application #
3274487
Study Section
(BET)
Project Start
1979-05-01
Project End
1990-06-30
Budget Start
1988-05-01
Budget End
1990-06-30
Support Year
19
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Illinois Urbana-Champaign
Department
Type
Schools of Arts and Sciences
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820

  Publications

Schmidt, Joann; Kuhlenschmidt, Mark S (2008) Microbial adhesion of Cryptosporidium parvum: identification of a colostrum-derived inhibitory lipid. Mol Biochem Parasitol 162:32-9
Kim, Sung Hoon; Katzenellenbogen, John A (2006) Hormone-PAMAM dendrimer conjugates: polymer dynamics and tether structure affect ligand access to receptors. Angew Chem Int Ed Engl 45:7243-8
Zhou, Hai-Bing; Comninos, John S; Stossi, Fabio et al. (2005) Synthesis and evaluation of estrogen receptor ligands with bridged oxabicyclic cores containing a diarylethylene motif: estrogen antagonists of unusual structure. J Med Chem 48:7261-74
Yang, Hui; Protiva, Petr; Gil, Roberto R et al. (2005) Antioxidant and cytotoxic isoprenylated coumarins from Mammea americana. Planta Med 71:852-60
De Angelis, Meri; Stossi, Fabio; Carlson, Kathryn A et al. (2005) Indazole estrogens: highly selective ligands for the estrogen receptor beta. J Med Chem 48:1132-44
Bigott, Heather M; Parent, Ephraim; Luyt, Leonard G et al. (2005) Design and synthesis of functionalized cyclopentadienyl tricarbonylmetal complexes for technetium-94m PET imaging of estrogen receptors. Bioconjug Chem 16:255-64
De Angelis, Meri; Stossi, Fabio; Waibel, Michael et al. (2005) Isocoumarins as estrogen receptor beta selective ligands: Isomers of isoflavone phytoestrogens and their metabolites. Bioorg Med Chem 13:6529-42
Baggett, Scott; Protiva, Petr; Mazzola, Eugene P et al. (2005) Bioactive benzophenones from Garcinia xanthochymus fruits. J Nat Prod 68:354-60
De Angelis, Meri; Katzenellenbogen, John A (2004) Ring nitrogen-substituted non-steroidal estrogens: pyridine and pyrimidine analogs of the phenol in deoxyhexestrol experience resonance constraints on preferred ligand conformation. Bioorg Med Chem Lett 14:5835-9
Sivaraman, Sharada; Sullivan, Todd J; Johnson, Francis et al. (2004) Inhibition of the bacterial enoyl reductase FabI by triclosan: a structure-reactivity analysis of FabI inhibition by triclosan analogues. J Med Chem 47:509-18

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