The long term objective of this project is an understanding of the way in which tRNA is biosynthesized and the contribution of specific structural features in tRNA to tRNA function. This is to be accomplished by the modification of tRNA's by chemical and enzymatic means to provide species that can help to define tRNA's processing and function at molecular level. For the next three-year period, tRNA's will be modified at the 3'-terminus to permit studies of the spatial requirements of the aminoacyl moieties of the A-site and P-site tRNA's during the peptidyltransferase reaction. Other modifications will be carried out by altering the size of the anticodon loop, to permit an assessment of the factors controlling codon-anticodon interaction, and to provide substrates for biosynthetic studies. A new approach to RNA sequencing is also proposed.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM027815-04A1
Application #
3275059
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1980-04-01
Project End
1987-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Virginia
Department
Type
Schools of Arts and Sciences
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
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Surratt, C K; Carter, B J; Payne, R C et al. (1990) Metal ion and substrate structure dependence of the processing of tRNA precursors by RNase P and M1 RNA. J Biol Chem 265:22513-9
Jager, A; Levy, M J; Hecht, S M (1988) Oligonucleotide N-alkylphosphoramidates: synthesis and binding to polynucleotides. Biochemistry 27:7237-46