The objectives of this research are to characterize the prosthetic group of the """"""""hemoprotein"""""""" myeloperoxidase, to then synthesize this heme and compare its properties to the native system in order to understand the mechanism of action of myeloperoxidase. The synthesis of vitamin B12 biosynthetic precursors Factors I, II and III is planned along with siroheme. The study of these and model isobacteriochlorins will be made in order to explain the mechanisms of action of the sulfite and nitrite reductases. Models for vitamin B12 will be synthesised and their chemistry, particularly that of the cobalt-carbon bond, will be studied in order to mimic and explain the mechanism of action of the vitamin B12 coenzyme. In addition, methods for the synthesis of pyrrolic, polypyrroles and pyrrolic macrocycles will be developed. Finally the organometallic chemistry of iron and model ruthenium porphyrins will be studied since such chemistry is involved in the misfunctioning of several hemeproteins such as hemoglobin, myoglobin and cytochrome P-450.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM029198-05
Application #
3276729
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1981-07-01
Project End
1987-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
5
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of British Columbia
Department
Type
DUNS #
800772162
City
Vancouver
State
BC
Country
Canada
Zip Code
V6 1Z3