Abstract

Ackerman et al. Recently demonstrated that high resolution nuclear magnetic resonance (NMR) spectroscopy may be conveniently performed in a sensitive, nondestructive and spatially selective manner on in vivo tissue by utilizing surface coils (Nature, 283, l67-170 (l980). The future implications for the fields of human biochemistry, physiology and medicine appear great. Pursuant to application in these areas the surface coil NMR (SC-NMR) methodology must be developed, critically evaluated, and applied to model animal systems. It is precisely these aims, the development, evaluation, and application of SC-NMR, which form the subject of this proposal. The impetus behind this program is the conviction that SC-NMR will evolve into a powerful probe of in vivo tissue biochemistry with a wide range of applications spanning the basic and clinical sciences. This proposed work seeks to explore and accurately define the potential of this novel spectroscopic approach. Preliminary SC-NMR studies proposed herein concern (i) Cardiovascular Applications, (ii) High-Energy Phosphates in Insulin Action, (iii) Mapping of the Nervous System, and (iv) Tumor Physiology and Pharamacology. These studies are designed to motivate and justify future applications with both animal and human subjects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
3R01GM030331-03S1
Application #
3278022
Study Section
(SSS)
Project Start
1982-06-01
Project End
1986-05-31
Budget Start
1984-12-10
Budget End
1986-05-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Arts and Sciences
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Miller, M I; Schaewe, T J; Bosch, C S et al. (1995) Model-based maximum-likelihood estimation for phase- and frequency-encoded magnetic-resonance-imaging data. J Magn Reson B 107:210-21
Henkelman, R M; Neil, J J; Xiang, Q S (1994) A quantitative interpretation of IVIM measurements of vascular perfusion in the rat brain. Magn Reson Med 32:464-9
Neil, J J; Bosch, C S; Ackerman, J J (1994) An evaluation of the sensitivity of the intravoxel incoherent motion (IVIM) method of blood flow measurement to changes in cerebral blood flow. Magn Reson Med 32:60-5
Neil, J J; Bretthorst, G L (1993) On the use of Bayesian probability theory for analysis of exponential decay data: an example taken from intravoxel incoherent motion experiments. Magn Reson Med 29:642-7
Bosch, C S; Ackerman, J J; Tilton, R G et al. (1993) In vivo NMR imaging and spectroscopic investigation of renal pathology in lean and obese rat kidneys. Magn Reson Med 29:335-44
Hotchkiss, R S; Rust, R S; Song, S K et al. (1993) Effect of sepsis on brain energy metabolism in normoxic and hypoxic rats. Circ Shock 40:303-10
Meeh, L A; Ackerman, J J; Thorpe, S R et al. (1992) Quantification of the accumulation and degradation of beta-very-low-density lipoproteins in vivo using a 19F-containing residualizing label and n.m.r. spectroscopy. Biochem J 286 ( Pt 3):785-92
Hwang, Y Y; Kim, S G; Evelhoch, J L et al. (1992) Nonglycolytic acidification of murine radiation-induced fibrosarcoma 1 tumor via 3-O-methyl-D-glucose monitored by 1H, 2H, 13C, and 31P nuclear magnetic resonance spectroscopy. Cancer Res 52:1259-66
Neil, J J; Song, S K; Ackerman, J J (1992) Concurrent quantification of tissue metabolism and blood flow via 2H/31P NMR in vivo. II. Validation of the deuterium NMR washout method for measuring organ perfusion. Magn Reson Med 25:56-66
Song, S K; Hotchkiss, R S; Ackerman, J J (1992) Concurrent quantification of tissue metabolism and blood flow via 2H/31P NMR in vivo. I. Assessment of absolute metabolite quantification. Magn Reson Med 25:45-55

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