Abstract

Continued support is requested for a project which will demonstrate the applicability of Fourier transform mass spectrometry to high resolution accurate mass analysis of ion with masses in the 1,500-20,000 dalton range. This will be accomplished using (1) laser desorption; (2) cesium ion bombardment; (3) fast atom bombardment; (4) thermospray techniques for sample volatilization in conjunction with a dual cell differentially-pumped Fourier transform mass spectrometer and an 8T magnet. It is expected that unprecedented high mass resolution at m/z 20,000 or above will be demonstrated. Furthermore, the relative sensitivity, accuracy, and resolution will be determined in comparison with results to be obtained with the same samples on a high performance VG Instruments ABZ-1HF double focussing mass spectrometer. The applicability of the methodologies developed to bipolymer analyses (e.g., polypeptides and polysugars) will be investigated.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM030604-04
Application #
3278389
Study Section
(BET)
Project Start
1982-06-01
Project End
1988-08-31
Budget Start
1985-09-19
Budget End
1986-08-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California Riverside
Department
Type
Earth Sciences/Resources
DUNS #
City
Riverside
State
CA
Country
United States
Zip Code
92521

  Publications

Ijames, C F; Wilkins, C L (1990) Surface-induced dissociation by Fourier transform mass spectrometry. Anal Chem 62:1295-9
Chowdhury, A K; Cooper, J R; Wilkins, C L (1989) Indium-alkene complex ions as reagents for selective chemical ionization. Anal Chem 61:86-8
Nuwaysir, L M; Wilkins, C L (1989) Photodissociation of laser-desorbed ions as a structure determination tool. Anal Chem 61:689-94
Fung, E T; Wilkins, C L (1988) Laser desorption/Fourier transform mass spectrometry of steroids. Biomed Environ Mass Spectrom 15:609-13
Nuwaysir, L M; Wilkins, C L (1988) Laser desorption Fourier transform mass spectrometry of polymers: comparison with secondary ion and fast atom bombardment mass spectrometry. Anal Chem 60:279-82
Rempel, D L; Ledford Jr, E B; Huang, S K et al. (1987) Parametric mode operation of a hyperbolic Penning trap for Fourier transform mass spectrometry. Anal Chem 59:2527-32
Coates, M L; Wilkins, C L (1987) Laser-desorption Fourier transform mass spectra of polysaccharides. Anal Chem 59:197-200
Coates, M L; Wilkins, C L (1986) Laser desorption/Fourier transform mass spectra of glycoalkaloids and steroid glycosides. Biomed Environ Mass Spectrom 13:199-204
Brown, R S; Wilkins, C L (1986) Laser-desorption Fourier transform mass spectrometry of synthetic porphyrins. Anal Chem 58:3196-9
Wilkins, C L; Weil, D A; Yang, C L et al. (1985) High mass analysis by laser desorption Fourier transform mass spectrometry. Anal Chem 57:520-4

Showing the most recent 10 out of 13 publications