The aim of the proposed research is to determine the gene structure of a major family of cell surface glycoproteins, collectively termed T200, which are present in different yet similar forms on the different lymphocyte lineages. Special emphasis will be directed toward determining how expression of the different forms of the molecules in this family is regulated. A genomic cloning strategy based on the ability of L cells to express T200 after transfection with DNA form lymphoid lines will be used to clone the T200 gene(s). The gene sequences encoding the different molecular forms will be compared with each other and the germline arrangement to determine whether gene rearrangement plays a role in the expression of these molecules. Using a genomic clone, the RNA and cDNA can be prepared for each of the different T200 forms and compared in order to detect any differences in RNA processing. The proposed research will also focus on molecular variations in T200 expression. Variations provided by evolution include the T200 genes of other species, including man, and the allelic forms of murine T200 genes. Additional variations have been selected in the laboratory and include structural gene mutants and revertants of these mutants. The goal of these studies will be to learn more about T200 expression and the function of the T200 glycoproteins. The gene clones will provide the means to investigate the change in T200 expression during differentiation in in vitro lymphocyte systems.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM032017-03
Application #
3280548
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1984-04-01
Project End
1987-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
009214214
City
La Jolla
State
CA
Country
United States
Zip Code
92037
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Zebedee, S L; Barritt, D; Epstein, R et al. (1991) Analysis of Ly5 chromosome 1 position using allelic differences and recombinant inbred mice. Eur J Immunogenet 18:155-63
Raschke, W C (1987) Cloned murine T200 (Ly-5) cDNA reveals multiple transcripts within B- and T-lymphocyte lineages. Proc Natl Acad Sci U S A 84:161-5
Raschke, W C; Hyman, R (1985) Stable expression of the mouse lymphocyte T200 antigen in L-cells after transfection with lymphoma DNA. Mol Immunol 22:1137-43