Abstract

A recently isolated maize transposable element, Mul, has been used to generate new mutants. We propose to use rapid screening methods to recover mutants deficient in one or more photosynthetic activities or groups of functionally related proteins. Further characterization of selected mutants will allow us to concentrate on those whose gene products appear to regulate the synthesis or assembly of photosynthetic complexes. Since the mutants were caused by Mul insertions, a Mul probe will be used to isolate mutants by molecular cloning. The role of the cloned genes in synthesis or assembly of multiprotein photosynthetic complexes will be determined.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM032760-02
Application #
3281852
Study Section
Genetics Study Section (GEN)
Project Start
1984-01-01
Project End
1986-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Type
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Tabas, J A; Zasloff, M; Wasmuth, J J et al. (1991) Bone morphogenetic protein: chromosomal localization of human genes for BMP1, BMP2A, and BMP3. Genomics 9:283-9
Taylor, W C; Barkan, A; Martienssen, R A (1987) Use of nuclear mutants in the analysis of chloroplast development. Dev Genet 8:305-20
Rock, C D; Barkan, A; Taylor, W C (1987) The maize plastid psbB-psbF-petB-petD gene cluster: spliced and unspliced petB and petD RNAs encode alternative products. Curr Genet 12:69-77