Fluorinated anesthetic agents (halothane, methoxyflurane, enflurane and isoflurane) incorporation into the brain of an intact rabbit will be monitored moninvasively with 19F NMR spectroscopy. High resolution 19F spectra of the listed anesthetic agents will be obtained with a surface coil centered over the animal calvarium on a TMR spectrometer equipped with a 20-cm bore superconducting magnet. The time-course of anesthetic uptake and elimination as well as the appearance of fluorine-containing metabolites in the brain will be followed with this technique for each of the four compounds. Nature of the environments these anesthetics occupy within the brain tissue will be inferred from the observed 19F chemical shifts. The in vivo results on intact animals will be confirmed by studies of excised brain samples. After isolation, identification and structural characterization of fluorinated compounds in the excised tissues will be accomplished with NMR spectroscopy and Mass spectrometry. 19F MNR spectroscopy will allow the first direct observation of volatile anesthetic agents in a brain of a live mammal, and make possible measurement of their uptake and elimination by this organ. The results obtained from this study will be used to assess the short and long-term effects on the cellular brain function resulting from anesthesia.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM033415-01
Application #
3283118
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1984-12-01
Project End
1987-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
Schools of Arts and Sciences
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
Venkatasubramanian, P N; Shen, Y J; Wyrwicz, A M (1995) In vivo 19F-NMR spectroscopic study of halothane uptake in rabbit brain. Biochim Biophys Acta 1245:262-8
Venkatasubramanian, P N; Shen, Y J; Wyrwicz, A M (1993) In vivo 19F one-dimensional chemical shift imaging study of isoflurane uptake in rabbit brain. NMR Biomed 6:377-82
Pouremad, R; Wyrwicz, A M (1991) Cerebral metabolism of fluorodeoxyglucose measured with 19F NMR spectroscopy. NMR Biomed 4:161-6
Wyrwicz, A M (1991) 19F in vivo NMR spectroscopy: a new technique to probe fluorinated anesthetic-tissue interactions. Ann N Y Acad Sci 625:733-42
Wyrwicz, A M; Conboy, C B (1989) Determination of halothane distribution in the rat head using 19F NMR technique. Magn Reson Med 9:219-28
Wyrwicz, A M; Conboy, C B; Ryback, K R et al. (1987) In vivo 19F-NMR study of isoflurane elimination from brain. Biochim Biophys Acta 927:86-91
Wyrwicz, A M; Conboy, C B; Nichols, B G et al. (1987) In vivo 19F-NMR study of halothane distribution in brain. Biochim Biophys Acta 929:271-7