The objectives of the proposed research project are the continued design, development, and evaluation of Fourier ion cyclotron resonance (FT-ICR) based techniques for the analysis and characterization of thermally labile biomolecules. During the past three to five years, major progress has been make in the development of high mass FT-ICR. However, certain aspects of the experimental results reveal specific problems which we attribute to trapping and/or detection of large (> m/z 2500-3000) ionic systems using FT-ICR. Probing and understanding these problems is further complicated by the fact that existing FT_ICR systems are not capable of making absolute measurements. That is, the signals measured give relative abundance of the ions trapped in the ion cell, but it is difficult to relate relative abundance measurements to absolute numbers of ions. Recent work has shown that ion detection is strongly dependent upon the initial ion motion, thus ion detection strongly discriminates against ions which possess non-thermal kinetic energies and which are initially out-of-phase with respect to the rf excitation. Absolute measurements are important because sensitivity and mass resolution are directly dependent upon the numbers of ions trapped in the ion cell. As a first approach to addressing issues related to trapping and detection efficiency of FT-ICR we have constructed a prototype time-of- flight/FT-ICR apparatus. This instrument will be used to investigate various aspects of trapping and detection of high mass (m/z 2500-15000) organic ions. An integral part of this instrument is the interface between the time-of-flight and FT-ICR instruments, e.g., the two-dimensional quadrupole lens assembly. This lensing element is potentially useful in may areas of FT-ICR, and especially on instruments utilizing external ion sources. In the later stages of the program, the two-dimensional lens assembly will be used to evaluate the use of different ion sources and structural probe methods for analyzing and characterizing large peptides and related systems.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM033780-05
Application #
3283787
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1984-12-01
Project End
1992-11-30
Budget Start
1990-12-01
Budget End
1991-11-30
Support Year
5
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Texas A&M University
Department
Type
Schools of Arts and Sciences
DUNS #
City
College Station
State
TX
Country
United States
Zip Code
77845
Preston, L M; Murray, K K; Russell, D H (1993) Reproducibility and quantitation of matrix-assisted laser desorption ionization mass spectrometry: effects of nitrocellulose on peptide ion yields. Biol Mass Spectrom 22:544-50
Solouki, T; Russell, D H (1992) Laser desorption studies of high mass biomolecules in Fourier-transform ion cyclotron resonance mass spectrometry. Proc Natl Acad Sci U S A 89:5701-4
Hanson, C D; Kerley, E L; Russell, D H (1989) High-resolution ion partitioning technique by phase-specific ion excitation for Fourier transform ion cyclotron resonance. Anal Chem 61:83-5
Hanson, C D; Castro, M E; Russel, D H (1989) Phase synchronization of an ion ensemble by frequency sweep excitation in Fourier transform ion cyclotron resonance. Anal Chem 61:2130-6
Hanson, C D; Kerley, E L; Castro, M E et al. (1989) Ion detection by Fourier transform ion cyclotron resonance: the effect of initial radial velocity on the coherent ion packet. Anal Chem 61:2040-6
Russell, D H; McGlohon, E S; Mallis, L M (1988) Fast-atom bombardment tandem mass spectrometry studies of organo-alkali-metal ions of small peptides. Competitive interaction of sodium with basic amino acid substituents. Anal Chem 60:1818-24
Mallis, L M; Raushel, F M; Russell, D H (1987) Differentiation of isotopically labeled nucleotides using fast atom bombardment tandem mass spectrometry. Anal Chem 59:980-4
Amster, I J; McLafferty, F W; Castro, M E et al. (1986) Detection of mass 16 241 ions by Fourier-transform mass spectrometry. Anal Chem 58:483-5
Mallis, L M; Russell, D H (1986) Fast atom bombardment--tandem mass spectrometry studies of organo-alkali metal ions of small peptides. Anal Chem 58:1076-80
Hilscher, L W; Hanson, C D; Russell, D H et al. (1985) Measurement of positional isotope exchange rates in enzyme-catalyzed reactions by fast atom bombardment mass spectrometry: application to argininosuccinate synthetase. Biochemistry 24:5888-93

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