Abstract

This project involves the determination of structure/function and biosynthesis of a membrane-bound lipo-peptidophosphogalactomannan, lipo-pPGM, from a species of Penicillium. Work proposed for the coming year is divided into three categories: 1) characterization of lipo-pPGM and comparing its properties to those of pPGM, 2) isolation and purification of the enzyme responsible for the removal of lipid residue(s) from lipo-pPGM, and 3) characterization of the products of the NAD:nucleoside diphosphate sugar oxidoreductase catalyzed reaction when UDP-D-glucose, UDP-D-galactose or GDP-D-mannose is the substrate being oxidized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034068-03
Application #
3284502
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1984-01-01
Project End
1987-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Florida
Department
Type
Earth Sciences/Resources
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611

  Publications

Abbas, C A; Groves, S; Gander, J E (1989) Isolation, purification, and properties of Penicillium charlesii alkaline protease. J Bacteriol 171:5630-7