Abstract

We have recently succeeded in isolating the replicative origin of the Escherichia coli chromosome. The complex is composed of the DNA at and near the origin, and various proteins. We call this complex OC, for """"""""origin complex"""""""". We will attempt further characterization of the OC particles. specifically, we will purify them with regard to protein content, and attempt to characterize the intrinsic proteins of these particles. Preliminary results have shown that partially purified DNA from OC binds to outer membrane, while other DNA does not. We will use a filter-binding assay to determine further the specificity and other properties of this interaction.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034132-16
Application #
3284660
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1984-07-01
Project End
1987-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
16
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Polaczek, P; Kwan, K; Liberies, D A et al. (1997) Role of architectural elements in combinatorial regulation of initiation of DNA replication in Escherichia coli. Mol Microbiol 26:261-75
von Freiesleben, U; Rasmussen, K V; Schaechter, M (1994) SeqA limits DnaA activity in replication from oriC in Escherichia coli. Mol Microbiol 14:763-72
Polaczek, P (1990) Bending of the origin of replication of E. coli by binding of IHF at a specific site. New Biol 2:265-71