Abstract

An understanding of mitosis, its regulation and its mechanics is central to many areas of medicine having to do with cell growth and division, e.g., cancer, embryological development and human genetics. The objective of this project is a better understanding of the mechanics and regulation of nuclear division. The immediate goal is to identify and characterize the products of specific genes involved in nuclear division by using DNA mediated transformation to complement specific mitotic mutations in Aspergillus nidulans. Genes will be cloned, sequenced and their products characterized by hybrid selection and translation of complementary messenger RNA's. Antibodies to the protein products of these genes will be prepared and used to localize the gene products in cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034711-02
Application #
3286170
Study Section
Genetics Study Section (GEN)
Project Start
1985-08-01
Project End
1990-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Medicine & Dentistry of NJ
Department
Type
Schools of Medicine
DUNS #
622146454
City
Piscataway
State
NJ
Country
United States
Zip Code
08854

  Publications

Lies, C M; Cheng, J; James, S W et al. (1998) BIMAAPC3, a component of the Aspergillus anaphase promoting complex/cyclosome, is required for a G2 checkpoint blocking entry into mitosis in the absence of NIMA function. J Cell Sci 111 ( Pt 10):1453-65
Xiang, X; Osmani, A H; Osmani, S A et al. (1995) Analysis of nuclear migration in Aspergillus nidulans. Cold Spring Harb Symp Quant Biol 60:813-9
Beckwith, S M; Roghi, C H; Morris, N R (1995) The genetics of nuclear migration in fungi. Genet Eng (N Y) 17:165-80
Chiu, Y H; Morris, N R (1995) Extragenic suppressors of nudC3, a mutation that blocks nuclear migration in Aspergillus nidulans. Genetics 141:453-64
Willins, D A; Xiang, X; Morris, N R (1995) An alpha tubulin mutation suppresses nuclear migration mutations in Aspergillus nidulans. Genetics 141:1287-98
James, S W; Mirabito, P M; Scacheri, P C et al. (1995) The Aspergillus nidulans bimE (blocked-in-mitosis) gene encodes multiple cell cycle functions involved in mitotic checkpoint control and mitosis. J Cell Sci 108 ( Pt 11):3485-99
Osmani, A H; van Peij, N; Mischke, M et al. (1994) A single p34cdc2 protein kinase (encoded by nimXcdc2) is required at G1 and G2 in Aspergillus nidulans. J Cell Sci 107 ( Pt 6):1519-28
O'Connell, M J; Meluh, P B; Rose, M D et al. (1993) Suppression of the bimC4 mitotic spindle defect by deletion of klpA, a gene encoding a KAR3-related kinesin-like protein in Aspergillus nidulans. J Cell Biol 120:153-62
Mirabito, P M; Morris, N R (1993) BIMA, a TPR-containing protein required for mitosis, localizes to the spindle pole body in Aspergillus nidulans. J Cell Biol 120:959-68
Morris, N R; Enos, A P (1992) Mitotic gold in a mold: Aspergillus genetics and the biology of mitosis. Trends Genet 8:32-7

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