Abstract

The proposed research is aimed at determining cellular pH by non-invasive, continuous measurement using 19F nuclear magnetic resonance. A variety of fluorinated molecules will be synthesized and tested in order to find optimal indicators and indicator precursors for measuring organelle and cytosolic pH under physiological conditions. As these methods are developed we will apply them to cytosolic and compartment pH in situ in isolated cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036433-05
Application #
3290406
Study Section
Biophysical Chemistry Study Section (BBCB)
Project Start
1986-04-01
Project End
1994-11-30
Budget Start
1991-12-01
Budget End
1992-11-30
Support Year
5
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Bental, M; Deutsch, C (1994) On-line studies of activation events in primary human T lymphocytes. Immunomethods 4:148-62
Bental, M; Deutsch, C (1994) 19F-NMR study of primary human T lymphocyte activation: effects of mitogen on intracellular pH. Am J Physiol 266:C541-51
Bental, M; Deutsch, C (1993) Metabolic changes in activated T cells: an NMR study of human peripheral blood lymphocytes. Magn Reson Med 29:317-26
Deutsch, C J; Taylor, J S (1989) New class of 19F pH indicators: fluoroanilines. Biophys J 55:799-804
Taylor, J; Deutsch, C (1988) 19F-nuclear magnetic resonance: measurements of [O2] and pH in biological systems. Biophys J 53:227-33
Deutsch, C J; Taylor, J S (1987) Intracellular pH as measured by 19F NMR. Ann N Y Acad Sci 508:33-47