The goal of this project is to characterize the role of amino acid compartmentation in the control of arginine metabolism in Neurospora crassa. Arginine and its biosynthetic and catabolic intermediate, ornithine, are sequestered in the vacuoles in this organism. The specificity, kinetics, energetics and regulation of amino acid transport across the vacuolar membrane and its relation to plasma membrane transport systems will be investigated. The effect of environmental alterations on vacuolar pH and the association of vacuolar arginine with polyphosphates will be investigated using in situ 15N-nuclear magnetic resonance spectroscopy. The relationship between nitrogen metabolite regulation and mobilization of vacuolar arginine will be investigated using mutants affecting nitrogen metabolite regulation. Finally, attempts will be made to isolate and characterize mutants impaired in movement of amino acids between the cytoplasmic and vacuolar compartments. The results may provide insight into the function of amino acid compartmentation in eukaryotic cells and organisms.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036552-03
Application #
3290762
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1986-04-01
Project End
1989-03-31
Budget Start
1988-04-01
Budget End
1989-03-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Type
Schools of Arts and Sciences
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Sauvanet, Cécile; Garbett, Damien; Bretscher, Anthony (2015) The function and dynamics of the apical scaffolding protein E3KARP are regulated by cell-cycle phosphorylation. Mol Biol Cell 26:3615-27
Keenan, K A; Weiss, R L (1997) Characterization of vacuolar arginine uptake and amino acid efflux in Neurospora crassa using cupric ion to permeabilize the plasma membrane. Fungal Genet Biol 22:177-90
Drees, B; Brown, C; Barrell, B G et al. (1995) Tropomyosin is essential in yeast, yet the TPM1 and TPM2 products perform distinct functions. J Cell Biol 128:383-92
Gessert, S F; Kim, J H; Nargang, F E et al. (1994) A polyprotein precursor of two mitochondrial enzymes in Neurospora crassa. Gene structure and precursor processing. J Biol Chem 269:8189-203
Yu, Y G; Weiss, R L (1992) Arginine transport in mitochondria of Neurospora crassa. J Biol Chem 267:15491-5
Paek, Y L; Weiss, R L (1989) Identification of an arginine carrier in the vacuolar membrane of Neurospora crassa. J Biol Chem 264:7285-90
Borkovich, K A; Weiss, R L (1987) Relationship between two major immunoreactive forms of arginase in Neurospora crassa. J Bacteriol 169:5510-7
Wandinger-Ness, A U; Weiss, R L (1987) A single precursor protein for two separable mitochondrial enzymes in Neurospora crassa. J Biol Chem 262:5823-30
Borkovich, K A; Weiss, R L (1987) Purification and characterization of arginase from Neurospora crassa. J Biol Chem 262:7081-6