Abstract

Five projects involving DNA-DNA hybridization are proposed; the organisms to be studied are several species of Drosophila. This first project is to measure overall heterozygosity using homozygous strains. The second is to study the relative rate of evolutionary change in nuclear DNA compared to mitochondrial DNA. The third project is to calibrate results from DNA-DNA hybridizations with respect to percent base pair mismatch. The the fourth project is to compare levels of genetic differentiation among sibling species of several groups. The final proposed project is to develop the technique of DNA-DNA hybridization to study the relative rates of evolution of a variety of functional classes of DNA. While all the proposed work concerns Drosophila, if the methodology can be established for this one group it can be used on virtually any other organism.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036648-03
Application #
3291035
Study Section
Genetics Study Section (GEN)
Project Start
1986-04-01
Project End
1990-07-31
Budget Start
1988-04-01
Budget End
1990-07-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Arts and Sciences
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Anderson, C L; Carew, E A; Powell, J R (1993) Evolution of the Adh locus in the Drosophila willistoni group: the loss of an intron, and shift in codon usage. Mol Biol Evol 10:605-18
Besansky, N J; Powell, J R (1992) Reassociation kinetics of Anopheles gambiae (Diptera: Culicidae) DNA. J Med Entomol 29:125-8
Cronin, M A; Nelson, M E; Pac, D F (1991) Spatial heterogeneity of mitochondrial DNA and allozymes among populations of white-tailed deer and mule deer. J Hered 82:118-27
Besansky, N J (1990) A retrotransposable element from the mosquito Anopheles gambiae . Mol Cell Biol 10:863-71
Powell, J R; Caccone, A (1989) Intraspecific and interspecific genetic variation in Drosophila. Genome 31:233-8
Caccone, A; Amato, G D; Powell, J R (1988) Rates and patterns of scnDNA and mtDNA divergence within the Drosophila melanogaster subgroup. Genetics 118:671-83
Caccone, A; DeSalle, R; Powell, J R (1988) Calibration of the change in thermal stability of DNA duplexes and degree of base pair mismatch. J Mol Evol 27:212-6
Caccone, A; Amato, G D; Powell, J R (1987) Intraspecific DNA divergence in Drosophila: a study on parthenogenetic D. mercatorum. Mol Biol Evol 4:343-50