Termination of transcription is a control point that is frequently utilized in prokaryotes to regulate gene expression. However, the extent to which this is true in eukaryotes is not yet clear. At present, the termination system which is best understood in eukaryotes is that of RNA polymerase I, due largely to studies on the frog, Xenopus laevis, in our own laboratory and studies on the mouse in the laboratory of Ingrid Grummt. The sites of termination are known, various mutants of these sites are available, and good assay systems for termination have been developed both in vivo and vitro. Fractionation of the proteins involved in termination is underway and we have begun to propose testable models for the process. In this application we propose to continue studies of RNA polymerase I termination in Xenopus. The proteins involved in termination will be identified and purified using and in vitro termination system. The genes for these proteins will be cloned and characterized. The heart of the proposal then is to determine the molecular mechanism by which these components effect termination of transcription by RNA polymerase I.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM041792-04
Application #
3300202
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1989-12-01
Project End
1994-11-30
Budget Start
1992-12-01
Budget End
1993-11-30
Support Year
4
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
075524595
City
Seattle
State
WA
Country
United States
Zip Code
98109
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Lang, W H; Platt, T; Reeder, R H (1998) Escherichia coli rho factor induces release of yeast RNA polymerase II but not polymerase I or III. Proc Natl Acad Sci U S A 95:4900-5
Jeong, S W; Lang, W H; Reeder, R H (1996) The yeast transcription terminator for RNA polymerase I is designed to prevent polymerase slippage. J Biol Chem 271:16104-10
Jeong, S W; Lang, W H; Reeder, R H (1995) The release element of the yeast polymerase I transcription terminator can function independently of Reb1p. Mol Cell Biol 15:5929-36
Lang, W H; Reeder, R H (1995) Transcription termination of RNA polymerase I due to a T-rich element interacting with Reb1p. Proc Natl Acad Sci U S A 92:9781-5
Lang, W H; Morrow, B E; Ju, Q et al. (1994) A model for transcription termination by RNA polymerase I. Cell 79:527-34
Reeder, R H; Lang, W (1994) The mechanism of transcription termination by RNA polymerase I. Mol Microbiol 12:11-5