NMR Studies of Proteins and Protein-DNA Binding

Spicer, Leonard D.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Research Project (R01)
• Project #: 5R01GM041829-02
• Application #: 3300277
• Study Section: Molecular and Cellular Biophysics Study Section (BBCA)
• Project Start: 1989-04-01
• Project End: 1992-03-31
• Budget Start: 1990-04-01
• Budget End: 1991-03-31
• Support Year: 2
• Fiscal Year: 1990

Institution

• Name: Duke University
• Type: Schools of Medicine
• DUNS #: 071723621
• City: Durham
• State: NC
• Country: United States
• Zip Code: 27705

Publications

Hyre, D E; Spicer, L D (1995) Improved excitation pulse bandwidths using shaped pulses, with application to heteronuclear half filters in macromolecular NMR. J Magn Reson B 108:12-21

Hyre, D E; Spicer, L D (1995) Thermodynamic evaluation of binding interactions in the methionine repressor system of Escherichia coli using isothermal titration calorimetry. Biochemistry 34:3212-21

Kaufman, J; Spicer, L D; Siegel, L M (1993) Proton NMR of Escherichia coli sulfite reductase: the unligated hemeprotein subunit. Biochemistry 32:2853-67

Kaufman, J; Siegel, L M; Spicer, L D (1993) Proton NMR of Escherichia coli sulfite reductase: studies of the heme protein subunit with added ligands. Biochemistry 32:8782-91

Farmer 2nd, B T; Venters, R A; Spicer, L D et al. (1992) A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules. J Biomol NMR 2:195-202

Venters, R A; Calderone, T L; Spicer, L D et al. (1991) Uniform 13C isotope labeling of proteins with sodium acetate for NMR studies: application to human carbonic anhydrase II. Biochemistry 30:4491-4

Hoffman, D W; Venters, R A; Shedd, S F et al. (1990) Use of gel filtration in the preparation of biological fluids for magnetic resonance spectroscopy. Magn Reson Med 13:507-13