Dr. Howell proposes to characterize proteins required for exocytic vesicle budding from the trans-Goli network (TGN) and to dissect their function in the steps of the budding process: recruitment of coat components to the TGN membrane, budding and uncoating of the vesicles. Her hypothesis is that the coat structure used in exocytic vesicle budding from the TGN is the lace-like TGNC, and that p62 and p200 will be components of this coat.
Specific Aim 1 is biochemical dissection of the p62 complex and p200 function in exocytic vesicle budding in vitro and in vivo.
Specific Aim 2 is molecular analysis of p62 and p200.
Specific Aim 3 is defining the exocytic vesicle coat structure.
Specific Aim 4 is identification of additional components required for TGN vesicle budding.
| Marsh, Brad J (2007) Reconstructing mammalian membrane architecture by large area cellular tomography. Methods Cell Biol 79:193-220 |
| Snyder, Christopher M; Mardones, Gonzalo A; Ladinsky, Mark S et al. (2006) GMx33 associates with the trans-Golgi matrix in a dynamic manner and sorts within tubules exiting the Golgi. Mol Biol Cell 17:511-24 |
| Mogelsvang, Soren; Howell, Kathryn E (2006) Global approaches to study Golgi function. Curr Opin Cell Biol 18:438-43 |
| Mardones, Gonzalo A; Snyder, Christopher M; Howell, Kathryn E (2006) Cis-Golgi matrix proteins move directly to endoplasmic reticulum exit sites by association with tubules. Mol Biol Cell 17:525-38 |
| Thompson, Roger J; Akana, Hillary C S R; Finnigan, Claire et al. (2006) Anion channels transport ATP into the Golgi lumen. Am J Physiol Cell Physiol 290:C499-514 |
| Fouassier, Laura; Nichols, Matthew T; Gidey, Elizabeth et al. (2005) Protein kinase C regulates the phosphorylation and oligomerization of ERM binding phosphoprotein 50. Exp Cell Res 306:264-73 |
| Marsh, Brad J; Volkmann, Niels; McIntosh, J Richard et al. (2004) Direct continuities between cisternae at different levels of the Golgi complex in glucose-stimulated mouse islet beta cells. Proc Natl Acad Sci U S A 101:5565-70 |
| Wu, Christine C; MacCoss, Michael J; Mardones, Gonzalo et al. (2004) Organellar proteomics reveals Golgi arginine dimethylation. Mol Biol Cell 15:2907-19 |
| Wu, Christine C; MacCoss, Michael J; Howell, Kathryn E et al. (2004) Metabolic labeling of mammalian organisms with stable isotopes for quantitative proteomic analysis. Anal Chem 76:4951-9 |
| Mogelsvang, Soren; Marsh, Brad J; Ladinsky, Mark S et al. (2004) Predicting function from structure: 3D structure studies of the mammalian Golgi complex. Traffic 5:338-45 |
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