Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM046127-06
Application #
2183665
Study Section
Biochemistry Study Section (BIO)
Project Start
1991-04-01
Project End
1999-03-31
Budget Start
1996-04-01
Budget End
1997-03-31
Support Year
6
Fiscal Year
1996
Total Cost
Indirect Cost
Name
New England Biolabs, Inc.
Department
Type
DUNS #
066605403
City
Ipswich
State
MA
Country
United States
Zip Code
01938
Dong, Aiping; Zhou, Lan; Zhang, Xing et al. (2004) Structure of the Q237W mutant of HhaI DNA methyltransferase: an insight into protein-protein interactions. Biol Chem 385:373-9
Pradhan, Sriharsa; Kim, Gun-Do (2002) The retinoblastoma gene product interacts with maintenance human DNA (cytosine-5) methyltransferase and modulates its activity. EMBO J 21:779-88
Bacolla, A; Pradhan, S; Larson, J E et al. (2001) Recombinant human DNA (cytosine-5) methyltransferase. III. Allosteric control, reaction order, and influence of plasmid topology and triplet repeat length on methylation of the fragile X CGG.CCG sequence. J Biol Chem 276:18605-13
Cheng, X; Roberts, R J (2001) AdoMet-dependent methylation, DNA methyltransferases and base flipping. Nucleic Acids Res 29:3784-95
Margot, J B; Aguirre-Arteta, A M; Di Giacco, B V et al. (2000) Structure and function of the mouse DNA methyltransferase gene: Dnmt1 shows a tripartite structure. J Mol Biol 297:293-300
Pradhan, S; Roberts, R J (2000) Hybrid mouse-prokaryotic DNA (cytosine-5) methyltransferases retain the specificity of the parental C-terminal domain. EMBO J 19:2103-14
Pradhan, S; Bacolla, A; Wells, R D et al. (1999) Recombinant human DNA (cytosine-5) methyltransferase. I. Expression, purification, and comparison of de novo and maintenance methylation. J Biol Chem 274:33002-10
Bacolla, A; Pradhan, S; Roberts, R J et al. (1999) Recombinant human DNA (cytosine-5) methyltransferase. II. Steady-state kinetics reveal allosteric activation by methylated dna. J Biol Chem 274:33011-9
O'Gara, M; Zhang, X; Roberts, R J et al. (1999) Structure of a binary complex of HhaI methyltransferase with S-adenosyl-L-methionine formed in the presence of a short non-specific DNA oligonucleotide. J Mol Biol 287:201-9
Roberts, R J; Cheng, X (1998) Base flipping. Annu Rev Biochem 67:181-98

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