This project deals with the mechanisms that account for regulation of ribosomal RNA synthesis. The mammalian rDNA promoter consists of two elements: a core promoter (CPE) and an upstream element (UPE). These interact with at least two transcription factors: UBF and SL1. SL1 binds both UPE and CPE and is required for transcription in vitro. UBF binds the CPE and is necessary for maximum transcriptional activity in vitro. The working hypothesis holds that UBF bends the DNA so as to approximate the SF1 binding sites. Rb blocks activation by UBF and prevents interaction between UBF and SL1. The expression of UBF is regulated by growth stimulation and differentiation, and the activity of UBF is regulated by phosphorylation. This project focuses primarily on the regulation of UBF expression and activity and the role of these processes in regulation of rDNA transcription.
| Zhang, Xue-Qian; Qureshi, Anwer; Song, Jianliang et al. (2003) Phospholemman modulates Na+/Ca2+ exchange in adult rat cardiac myocytes. Am J Physiol Heart Circ Physiol 284:H225-33 |
| Flynn, Brian J; Mian, Hamayun S; Cera, Peter J et al. (2002) Early molecular changes in bladder hypertrophy due to bladder outlet obstruction. Urology 59:978-82 |
| Song, Jianliang; Zhang, Xue-Qian; Carl, Lois L et al. (2002) Overexpression of phospholemman alters contractility and [Ca(2+)](i) transients in adult rat myocytes. Am J Physiol Heart Circ Physiol 283:H576-83 |
| Zhang, Xue-Qian; Song, Jianliang; Qureshi, Anwer et al. (2002) Rescue of contractile abnormalities by Na+/Ca2+ exchanger overexpression in postinfarction rat myocytes. J Appl Physiol 93:1925-31 |
| Tadros, George M; Zhang, Xue-Qian; Song, Jianliang et al. (2002) Effects of Na(+)/Ca(2+) exchanger downregulation on contractility and [Ca(2+)](i) transients in adult rat myocytes. Am J Physiol Heart Circ Physiol 283:H1616-26 |
| Chu, Xin; Cheung, Joseph Y; Barber, Dwayne L et al. (2002) Erythropoietin modulates calcium influx through TRPC2. J Biol Chem 277:34375-82 |
| Stefanovsky, V Y; Pelletier, G; Hannan, R et al. (2001) An immediate response of ribosomal transcription to growth factor stimulation in mammals is mediated by ERK phosphorylation of UBF. Mol Cell 8:1063-73 |
| Zhang, X Q; Song, J; Rothblum, L I et al. (2001) Overexpression of Na+/Ca2+ exchanger alters contractility and SR Ca2+ content in adult rat myocytes. Am J Physiol Heart Circ Physiol 281:H2079-88 |
| Biggiogera, M; Malatesta, M; Abolhassani-Dadras, S et al. (2001) Revealing the unseen: the organizer region of the nucleolus. J Cell Sci 114:3199-205 |
| Hirschler-Laszkiewicz, I; Cavanaugh, A; Hu, Q et al. (2001) The role of acetylation in rDNA transcription. Nucleic Acids Res 29:4114-24 |
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