A novel characteristic of baculovirus infection is the unusual and abundant induction, synthesis and assembly of cellular membranes, particularly in the nucleoplasm. The molecular strategy utilized by the baculovirus to obtain an envelope is quite unique. De novo membrane assembly in the nucleoplasm has been hypothesized as the process for the elaborate induction of intranuclear membranes; however, this theory is not consistent with current models of eukaryotic cell membrane assembly and lipid biosynthesis. Our studies identify several PDV envelope proteins and one of these, PDV-E66, is present in intranuclear microvesicles and the viral envelope. This is the first biochemical evidence that the nuclear microvesicles function as a source or an intermediate of the PDV viral envelope. The identification of PDV envelope proteins provide markers to allow the biosynthesis, trafficking and assembly of membranes which occur in the infected cell nucleoplasm. Our observations and preliminary data suggest that the Autographa californica nuclear polyhidrosis baculovirus may encode homologs of key cellular proteins that may function to influence inner nuclear membrane activities and de novo lipid synthesis. This study may not only reveal the novel envelopment strategies utilized by AcMNPV but could also reveal new details of eukaryotic cellular pathways of transport and nuclear membrane biogenesis. AcMNPV has become the most extensively studied baculovirus because of its use as a cloning and expression vector and its use and development for crop protection as a natural or genetically engineered microbial pest control agent. The biology of baculovirus-host interactions is uniquely complex yet studies of this virus-hose system are serving an important role in the development of fundamental knowledge of molecular and biochemical pathways in insects and invertebrates for comparison with the more comprehensively studied vertebrate systems. These studies are relevant to the very significant role that invertebrates play in the transmission of disease to plants, animals and humans and for their use in pest control strategies fundamental to crop protection and public health.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM047552-06
Application #
2415166
Study Section
Experimental Virology Study Section (EVR)
Project Start
1992-05-01
Project End
1999-04-30
Budget Start
1997-05-01
Budget End
1998-04-30
Support Year
6
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Texas Agrilife Research
Department
Zoology
Type
Schools of Earth Sciences/Natur
DUNS #
110521739
City
College Station
State
TX
Country
United States
Zip Code
77843
Braunagel, S C; Guidry, P A; Rosas-Acosta, G et al. (2001) Identification of BV/ODV-C42, an Autographa californica nucleopolyhedrovirus orf101-encoded structural protein detected in infected-cell complexes with ODV-EC27 and p78/83. J Virol 75:12331-8
Rosas-Acosta, G; Braunagel, S C; Summers, M D (2001) Effects of deletion and overexpression of the Autographa californica nuclear polyhedrosis virus FP25K gene on synthesis of two occlusion-derived virus envelope proteins and their transport into virus-induced intranuclear membranes. J Virol 75:10829-42
Braunagel, S C; Burks, J K; Rosas-Acosta, G et al. (1999) Mutations within the Autographa californica nucleopolyhedrovirus FP25K gene decrease the accumulation of ODV-E66 and alter its intranuclear transport. J Virol 73:8559-70
Beniya, H; Braunagel, S C; Summers, M D (1998) Autographa californica nuclear polyhedrosis virus: subcellular localization and protein trafficking of BV/ODV-E26 to intranuclear membranes and viral envelopes. Virology 240:64-75
Belyavskyi, M; Braunagel, S C; Summers, M D (1998) The structural protein ODV-EC27 of Autographa californica nucleopolyhedrovirus is a multifunctional viral cyclin. Proc Natl Acad Sci U S A 95:11205-10
Braunagel, S C; Parr, R; Belyavskyi, M et al. (1998) Autographa californica nucleopolyhedrovirus infection results in Sf9 cell cycle arrest at G2/M phase. Virology 244:195-211
Hong, T; Summers, M D; Braunagel, S C (1997) N-terminal sequences from Autographa californica nuclear polyhedrosis virus envelope proteins ODV-E66 and ODV-E25 are sufficient to direct reporter proteins to the nuclear envelope, intranuclear microvesicles and the envelope of occlusion derived virus. Proc Natl Acad Sci U S A 94:4050-5
Braunagel, S C; Elton, D M; Ma, H et al. (1996) Identification and analysis of an Autographa californica nuclear polyhedrosis virus structural protein of the occlusion-derived virus envelope: ODV-E56. Virology 217:97-110
Harrison, R L; Jarvis, D L; Summers, M D (1996) The role of the AcMNPV 25K gene, ""FP25,"" in baculovirus polh and p10 expression. Virology 226:34-46
Braunagel, S C; He, H; Ramamurthy, P et al. (1996) Transcription, translation, and cellular localization of three Autographa californica nuclear polyhedrosis virus structural proteins: ODV-E18, ODV-E35, and ODV-EC27. Virology 222:100-14

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