Abstract

The long term goal of this work is to develop lower cost analytical instrumentation and the associated analytical methodology for improved structure determination of biomolecules. Matrix-assisted laser desorption ionization (MALDI) will be combined with a quadrupole ion trap. The MALDI technique appears to have a number of characteristics that will make it superior to the standard method ionization method for biological molecules in use today, Fast Atom Bombardment (FAB). MALDI has the potential to have significantly better sensitivity than FAB, is less prone to matrix/sample incompatibilities, and produces significantly less chemical noise in the mass spectrum. The quadrupole ion trap has become well known for its sensitivity and MS/MS capabilities and thus the combination of it with MALDI should offer tremendous potential. In addition to optimizing the interface of MALDI with the ion trap, work will be performed to better understand the physico-chemical aspects of MS/MS of ions from biological molecules.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM049852-05A2
Application #
2857184
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1993-08-01
Project End
2003-03-31
Budget Start
1999-04-01
Budget End
2000-03-31
Support Year
5
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Bushey, Jared M; Kaplan, Desmond A; Danell, Ryan M et al. (2009) Pulsed Nano-Electrospray Ionization: Characterization of Temporal Response and Implementation with a Flared Inlet Capillary. Instrum Sci Technol 37:257-273
Black, David M; Payne, Anne H; Glish, Gary L (2006) Determination of cooling rates in a quadrupole ion trap. J Am Soc Mass Spectrom 17:932-8
Payne, Anne H; Glish, Gary L (2005) Tandem mass spectrometry in quadrupole ion trap and ion cyclotron resonance mass spectrometers. Methods Enzymol 402:109-48
Danell, Ryan M; Danell, Allison S; Glish, Gary L et al. (2003) The use of static pressures of heavy gases within a quadrupole ion trap. J Am Soc Mass Spectrom 14:1099-109
Asam, Michael R; Glish, Gary L (2002) Collision-induced signal enhancement (CISE): the use of boundary activation to effect non-resonant CISE. J Am Soc Mass Spectrom 13:650-8
Lin, T; Payne, A H; Glish, G L (2001) Dissociation pathways of alkali-cationized peptides: opportunities for C-terminal peptide sequencing. J Am Soc Mass Spectrom 12:497-504
Payne, A H; Glish, G L (2001) Thermally assisted infrared multiphoton photodissociation in a quadrupole ion trap. Anal Chem 73:3542-8
Danell, A S; Glish, G L (2001) Evidence for ionization-related conformational differences of peptide ions in a quadrupole ion trap. J Am Soc Mass Spectrom 12:1331-8
Payne, A H; Chelf, J H; Glish, G L (2000) C-terminal peptide sequencing using acetylated peptides with MSn in a quadrupole ion trap. Analyst 125:635-40
Asam, M R; Glish, G L (1999) Determination of the dissociation kinetics of a transient intermediate. J Am Soc Mass Spectrom 10:119-25

Showing the most recent 10 out of 17 publications