Structure/Mechanism of 6-Phosphogluconate Dehydrogenase

Cook, Paul F.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Research Project (R01)
• Project #: 5R01GM050872-02
• Application #: 2189020
• Study Section: Biochemistry Study Section (BIO)
• Project Start: 1994-12-01
• Project End: 1996-07-31
• Budget Start: 1995-12-01
• Budget End: 1996-07-31
• Support Year: 2
• Fiscal Year: 1996

Institution

• Name: University of North Texas
• Department: Biochemistry
• Type: Schools of Osteopathy
• DUNS #: 110091808
• City: Fort Worth
• State: TX
• Country: United States
• Zip Code: 76107

Publications

Zhang, L; Chooback, L; Cook, P F (1999) Lysine 183 is the general base in the 6-phosphogluconate dehydrogenase-catalyzed reaction. Biochemistry 38:11231-8

Karsten, W E; Chooback, L; Cook, P F (1998) Glutamate 190 is a general acid catalyst in the 6-phosphogluconate-dehydrogenase-catalyzed reaction. Biochemistry 37:15691-7

Hwang, C C; Cook, P F (1998) Multiple isotope effects as a probe of proton and hydride transfer in the 6-phosphogluconate dehydrogenase reaction. Biochemistry 37:15698-702

Chooback, L; Price, N E; Karsten, W E et al. (1998) Cloning, expression, purification, and characterization of the 6-phosphogluconate dehydrogenase from sheep liver. Protein Expr Purif 13:251-8

Hwang, C C; Berdis, A J; Karsten, W E et al. (1998) Oxidative decarboxylation of 6-phosphogluconate by 6-phosphogluconate dehydrogenase proceeds by a stepwise mechanism with NADP and APADP as oxidants. Biochemistry 37:12596-602

Price, N E; Cook, P F (1996) Kinetic and chemical mechanisms of the sheep liver 6-phosphogluconate dehydrogenase. Arch Biochem Biophys 336:215-23