Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM051194-03
Application #
2189545
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1994-08-01
Project End
1999-07-31
Budget Start
1996-08-01
Budget End
1997-07-31
Support Year
3
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Utah
Department
Biochemistry
Type
Schools of Medicine
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Stray, James E; Crisona, Nancy J; Belotserkovskii, Boris P et al. (2005) The Saccharomyces cerevisiae Smc2/4 condensin compacts DNA into (+) chiral structures without net supercoiling. J Biol Chem 280:34723-34
Stray, James E; Lindsley, Janet E (2003) Biochemical analysis of the yeast condensin Smc2/4 complex: an ATPase that promotes knotting of circular DNA. J Biol Chem 278:26238-48
Lindsley, J E (2001) Use of a real-time, coupled assay to measure the ATPase activity of DNA topoisomerase II. Methods Mol Biol 95:57-64
Baird, C L; Gordon, M S; Andrenyak, D M et al. (2001) The ATPase reaction cycle of yeast DNA topoisomerase II. Slow rates of ATP resynthesis and P(i) release. J Biol Chem 276:27893-8
Morris, S K; Baird, C L; Lindsley, J E (2000) Steady-state and rapid kinetic analysis of topoisomerase II trapped as the closed-clamp intermediate by ICRF-193. J Biol Chem 275:2613-8
Morris, S K; Lindsley, J E (1999) Yeast topoisomerase II is inhibited by etoposide after hydrolyzing the first ATP and before releasing the second ADP. J Biol Chem 274:30690-6
Lindsley, J E (1999) Overexpression and purification of Saccharomyces cerevisiae DNA topoisomerase II from yeast. Methods Mol Biol 94:187-97
Baird, C L; Harkins, T T; Morris, S K et al. (1999) Topoisomerase II drives DNA transport by hydrolyzing one ATP. Proc Natl Acad Sci U S A 96:13685-90
Morris, S K; Harkins, T T; Tennyson, R B et al. (1999) Kinetic and thermodynamic analysis of mutant type II DNA topoisomerases that cannot covalently cleave DNA. J Biol Chem 274:3446-52
Harkins, T T; Lindsley, J E (1998) Pre-steady-state analysis of ATP hydrolysis by Saccharomyces cerevisiae DNA topoisomerase II. 1. A DNA-dependent burst in ATP hydrolysis. Biochemistry 37:7292-8

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