Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM053923-01
Application #
2193322
Study Section
Genome Study Section (GNM)
Project Start
1996-05-01
Project End
2000-04-30
Budget Start
1996-05-01
Budget End
1997-04-30
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Public Health & Prev Medicine
Type
Schools of Public Health
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Chandrasegaran, Srinivasan; Carroll, Dana (2016) Origins of Programmable Nucleases for Genome Engineering. J Mol Biol 428:963-89
Mani, Mala; Kandavelou, Karthikeyan; Dy, Fei Jamie et al. (2005) Design, engineering, and characterization of zinc finger nucleases. Biochem Biophys Res Commun 335:447-57
Mani, Mala; Smith, Jeff; Kandavelou, Karthikeyan et al. (2005) Binding of two zinc finger nuclease monomers to two specific sites is required for effective double-strand DNA cleavage. Biochem Biophys Res Commun 334:1191-7
Choe, Wonchae; Chandrasegaran, Srinivasan; Ostermeier, Marc (2005) Protein fragment complementation in M.HhaI DNA methyltransferase. Biochem Biophys Res Commun 334:1233-40
Durai, Sundar; Mani, Mala; Kandavelou, Karthikeyan et al. (2005) Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells. Nucleic Acids Res 33:5978-90
Bibikova, M; Carroll, D; Segal, D J et al. (2001) Stimulation of homologous recombination through targeted cleavage by chimeric nucleases. Mol Cell Biol 21:289-97
Ruminy, P; Derambure, C; Chandrasegaran, S et al. (2001) Long-range identification of hepatocyte nuclear factor-3 (FoxA) high and low-affinity binding sites with a chimeric nuclease. J Mol Biol 310:523-35
Smith, J; Bibikova, M; Whitby, F G et al. (2000) Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains. Nucleic Acids Res 28:3361-9
Smith, J; Berg, J M; Chandrasegaran, S (1999) A detailed study of the substrate specificity of a chimeric restriction enzyme. Nucleic Acids Res 27:674-81
Chandrasegaran, S; Smith, J (1999) Chimeric restriction enzymes: what is next? Biol Chem 380:841-8

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