Molecular Basis of Human Cytochrome P450 3a Function

Halpert, James R.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Research Project (R01)
• Project #: 5R01GM054995-02
• Application #: 2655020
• Study Section: Pharmacology A Study Section (PHRA)
• Project Start: 1997-02-01
• Project End: 1998-08-31
• Budget Start: 1998-02-01
• Budget End: 1998-08-31
• Support Year: 2
• Fiscal Year: 1998

Institution

• Name: University of Arizona
• Department: Pharmacology
• Type: Schools of Pharmacy
• City: Tucson
• State: AZ
• Country: United States
• Zip Code: 85721

Publications

Davydov, Dmitri R; Yang, Zhongyu; Davydova, Nadezhda et al. (2016) Conformational Mobility in Cytochrome P450 3A4 Explored by Pressure-Perturbation EPR Spectroscopy. Biophys J 110:1485-1498

Davydov, Dmitri R; Davydova, Nadezhda Y; Sineva, Elena V et al. (2015) Interactions among cytochromes P450 in microsomal membranes: oligomerization of cytochromes P450 3A4, 3A5, and 2E1 and its functional consequences. J Biol Chem 290:3850-64

Müller, Christian S; Knehans, Tim; Davydov, Dmitri R et al. (2015) Concurrent cooperativity and substrate inhibition in the epoxidation of carbamazepine by cytochrome P450 3A4 active site mutants inspired by molecular dynamics simulations. Biochemistry 54:711-21

Davydov, Dmitri R; Sineva, Elena V; Davydova, Nadezhda Y et al. (2013) CYP261 enzymes from deep sea bacteria: a clue to conformational heterogeneity in cytochromes P450. Biotechnol Appl Biochem 60:30-40

Sineva, Elena V; Rumfeldt, Jessica A O; Halpert, James R et al. (2013) A large-scale allosteric transition in cytochrome P450 3A4 revealed by luminescence resonance energy transfer (LRET). PLoS One 8:e83898

Davydov, Dmitri R; Davydova, Nadezhda Y; Sineva, Elena V et al. (2013) Pivotal role of P450-P450 interactions in CYP3A4 allostery: the case of ?-naphthoflavone. Biochem J 453:219-30

Davydov, Dmitri R; Rumfeldt, Jessica A O; Sineva, Elena V et al. (2012) Peripheral ligand-binding site in cytochrome P450 3A4 located with fluorescence resonance energy transfer (FRET). J Biol Chem 287:6797-809

Shimshoni, Jakob A; Roberts, Arthur G; Scian, Michele et al. (2012) Stereoselective formation and metabolism of 4-hydroxy-retinoic Acid enantiomers by cytochrome p450 enzymes. J Biol Chem 287:42223-32

Roberts, Arthur G; Yang, Jing; Halpert, James R et al. (2011) The structural basis for homotropic and heterotropic cooperativity of midazolam metabolism by human cytochrome P450 3A4. Biochemistry 50:10804-18

Fernando, Harshica; Rumfeldt, Jessica A O; Davydova, Nadezhda Y et al. (2011) Multiple substrate-binding sites are retained in cytochrome P450 3A4 mutants with decreased cooperativity. Xenobiotica 41:281-9