Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
9R01GM055430-06
Application #
2194309
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Project Start
1996-07-01
Project End
2000-06-30
Budget Start
1996-07-01
Budget End
1997-06-30
Support Year
6
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Tufts University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02111
Sapunaric, Frederic M; Levy, Stuart B (2007) Interdomain loop mutation Asp190Cys of the tetracycline efflux transporter TetA(B) decreases affinity for substrate. Antimicrob Agents Chemother 51:3036-7
Sapunaric, Frederic M; Levy, Stuart B (2005) Substitutions in the interdomain loop of the Tn10 TetA efflux transporter alter tetracycline resistance and substrate specificity. Microbiology 151:2315-22
Sapunaric, Frederic M; Levy, Stuart B (2003) Second-site suppressor mutations for the serine 202 to phenylalanine substitution within the interdomain loop of the tetracycline efflux protein Tet(C). J Biol Chem 278:28588-92
McMurry, Laura M; Aldema-Ramos, Mila L; Levy, Stuart B (2002) Fe(2+)-tetracycline-mediated cleavage of the Tn10 tetracycline efflux protein TetA reveals a substrate binding site near glutamine 225 in transmembrane helix 7. J Bacteriol 184:5113-20
Yin, C C; Aldema-Ramos, M L; Borges-Walmsley, M I et al. (2000) The quarternary molecular architecture of TetA, a secondary tetracycline transporter from Escherichia coli. Mol Microbiol 38:482-92
Saraceni-Richards, C A; Levy, S B (2000) Second-site suppressor mutations of inactivating substitutions at gly247 of the tetracycline efflux protein, Tet(B). J Bacteriol 182:6514-6
Saraceni-Richards, C A; Levy, S B (2000) Evidence for interactions between helices 5 and 8 and a role for the interdomain loop in tetracycline resistance mediated by hybrid Tet proteins. J Biol Chem 275:6101-6
Levy, S B; McMurry, L M; Barbosa, T M et al. (1999) Nomenclature for new tetracycline resistance determinants. Antimicrob Agents Chemother 43:1523-4
Kennan, R M; McMurry, L M; Levy, S B et al. (1997) Glutamate residues located within putative transmembrane helices are essential for TetA(P)-mediated tetracycline efflux. J Bacteriol 179:7011-5