The studies outlined in this proposal are designed to develop a better understanding of the determinants of efficient ribozyme cleavage of HIV-1 RNAs.
The specific aims of this proposal are: 1) To determine if models of the secondary structure of HIV-1 mRNAs predict efficient ribozyme cleavage of specific sites within these RNAs. 2) To determine if intracellular localization of target RNAs affects ribozyme activity. and 3) To determine if intracellular localization of ribozymes affects ribozyme catalytic activity. Although the research plan outlined in this proposal is designed to evaluate the factors that affect ribozyme-substrate interactions in cells, these studies are expected to contribute to a better understanding of intracellular RNA-RNA interactions in general and to help in the application of other RNA based technologies such as RNA ligands and antisense RNAs. In addition to the development of more effective anti-HIV therapies, the studies in this proposal may be applicable to the development of RNA-based therapies of other diseases in which modification or inhibition of gene expression could alter disease progression.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM055503-02
Application #
2701802
Study Section
AIDS and Related Research Study Section 4 (ARRD)
Project Start
1997-05-01
Project End
2000-04-30
Budget Start
1998-05-01
Budget End
1999-04-30
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045