Abstract

The motility of endothelial cells will be studied. The effects of calcium and cAMP concentrations will be measured and related to wound-induced transcription of the c-fos and c-jun genes. The sufficiency and neccessesity of transcription of these genes for cell motility will be tested. Similarly the necessity and sufficiency of changes of calcium will be determined for fos and jun induction as well as cell motility.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
7R01GM056416-05
Application #
6650694
Study Section
Biological Sciences 2 (BIOL)
Project Start
1997-09-01
Project End
2003-07-31
Budget Start
2002-08-01
Budget End
2003-07-31
Support Year
5
Fiscal Year
2000
Total Cost
$54,720
Indirect Cost

  Institution

Name
Magee-Women's Research Institute and Foundation
Department
Type
DUNS #
058625146
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Schmidt, Nathan W; Mishra, Abhijit; Wang, Jun et al. (2013) Influenza virus A M2 protein generates negative Gaussian membrane curvature necessary for budding and scission. J Am Chem Soc 135:13710-9
Balannik, Victoria; Obrdlik, Petr; Inayat, Samsoon et al. (2010) Solid-supported membrane technology for the investigation of the influenza A virus M2 channel activity. Pflugers Arch 459:593-605
Balannik, Victoria; Carnevale, Vincenzo; Fiorin, Giacomo et al. (2010) Functional studies and modeling of pore-lining residue mutants of the influenza a virus M2 ion channel. Biochemistry 49:696-708
Wang, Jun; Cady, Sarah D; Balannik, Victoria et al. (2009) Discovery of spiro-piperidine inhibitors and their modulation of the dynamics of the M2 proton channel from influenza A virus. J Am Chem Soc 131:8066-76
Balannik, Victoria; Wang, Jun; Ohigashi, Yuki et al. (2009) Design and pharmacological characterization of inhibitors of amantadine-resistant mutants of the M2 ion channel of influenza A virus. Biochemistry 48:11872-82
Tran, P O; Hinman, L E; Unger, G M et al. (1999) A wound-induced [Ca2+]i increase and its transcriptional activation of immediate early genes is important in the regulation of motility. Exp Cell Res 246:319-26