1-H NMR techniques have detected the myoglobin signals in perfused myocardium and have opened a unique opportunity to investigate the fundamental question of oxygen regulation in tissue. How the cell responds to varying oxygen levels and meets the complex energy demand is central to a broad range of issues in cardiac/skeletal muscle physiology and metabolism. Perfused heart experiments have established the spectroscopic techniques to measure the intracellular oxygen in myocardium with the proximal histidyl NH and Val E11 signals of Mb and have set the stage for investigating the regulation of oxidative metabolism in the intact animal. They have also established a critical basis to measure the intracellular pO2 in blood perfused organs and to determine the regulation of respiration in the myocardium in situ. The proposal addresses a key question about the mechanism regulating respiration in myocardium. It will specifically assess whether the oxygen supply actually determines the respiration rate. Developing and utilizing the Mb technique to measure intracellular pO2 will then shape our understanding of oxidative phosphorylation in vivo.
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| Gros, Gerolf; Wittenberg, Beatrice A; Jue, Thomas (2010) Myoglobin's old and new clothes: from molecular structure to function in living cells. J Exp Biol 213:2713-25 |
| Xie, Hongtao; Kreutzer, Ulrike; Jue, Thomas (2009) Oximetry with the NMR signals of hemoglobin Val E11 and Tyr C7. Eur J Appl Physiol 107:325-33 |
| Sriram, Renuka; Kreutzer, Ulrike; Shih, Lifan et al. (2008) Interaction of fatty acid with myoglobin. FEBS Lett 582:3643-9 |
| Masuda, Kazumi; Truscott, Kent; Lin, Ping-Chang et al. (2008) Determination of myoglobin concentration in blood-perfused tissue. Eur J Appl Physiol 104:41-8 |
| Lin, Ping-Chang; Kreutzer, Ulrike; Jue, Thomas (2007) Myoglobin translational diffusion in rat myocardium and its implication on intracellular oxygen transport. J Physiol 578:595-603 |
| Lin, Ping-Chang; Kreutzer, Ulrike; Jue, Thomas (2007) Anisotropy and temperature dependence of myoglobin translational diffusion in myocardium: implication for oxygen transport and cellular architecture. Biophys J 92:2608-20 |
| Chung, Youngran; Huang, Shih-Jwo; Glabe, Alan et al. (2006) Implication of CO inactivation on myoglobin function. Am J Physiol Cell Physiol 290:C1616-24 |
| Kreutzer, Ulrike; Jue, Thomas (2006) Investigation of bioactive NO-scavenging role of myoglobin in myocardium. Pflugers Arch 452:36-42 |
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