Abstract

Nuclear envelope breakdown (NEBD) is an essential process of both the mitotic and meiotic cell cycle. The mechanisms regulating NEBD will be investigated by imaging the spatial and temporal dynamics of green fluorescent protein chimeras of cyclin, nuclear pore proteins, and lamin in living starfish oocytes. Starfish oocytes will be used because they are optically clear, and have a large nucleus that undergoes NEBD at a precisely defined time (20-30 min) after application of a hormone. Confocal microscopy and photobleaching techniques will be used to address three specific aims: 1) to investigate mechanisms by which cyclin B/cdc2 enters the nucleus, 2) to investigate the relationship between nuclear pore disassembly and the permeability increase that precedes NEBD, and 3) to investigate the mechanisms of disruption of the nuclear envelope membrane. These studies should provide information about the fundamental question of the regulation of the cell cycle.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM060389-05
Application #
6711744
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Deatherage, James F
Project Start
2000-03-01
Project End
2008-02-29
Budget Start
2004-03-01
Budget End
2008-02-29
Support Year
5
Fiscal Year
2004
Total Cost
$180,332
Indirect Cost

  Institution

Name
University of Connecticut
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
022254226
City
Farmington
State
CT
Country
United States
Zip Code
06030

  Publications

Terasaki, Mark; Runft, Linda (2010) Two-stage dependence for 1-methyladenine induced reinitiation of meiotic maturation in starfish oocytes. Exp Cell Res 316:2654-63
Terasaki, Mark (2006) Quantification of fluorescence in thick specimens, with an application to cyclin B-GFP expression in starfish oocytes. Biol Cell 98:245-52
Fein, Alan; Terasaki, Mark (2005) Rapid increase in plasma membrane chloride permeability during wound resealing in starfish oocytes. J Gen Physiol 126:151-9
Slepchenko, Boris M; Terasaki, Mark (2004) Bio-switches: what makes them robust? Curr Opin Genet Dev 14:428-34
Morris, Robert L; English, Christopher N; Lou, Julia E et al. (2004) Redistribution of the kinesin-II subunit KAP from cilia to nuclei during the mitotic and ciliogenic cycles in sea urchin embryos. Dev Biol 274:56-69
Slepchenko, Boris M; Terasaki, Mark (2003) Cyclin aggregation and robustness of bio-switching. Mol Biol Cell 14:4695-706
Galbraith, James A; Terasaki, Mark (2003) Controlled damage in thick specimens by multiphoton excitation. Mol Biol Cell 14:1808-17
Terasaki, Mark; Okumura, Ei-Ichi; Hinkle, Beth et al. (2003) Localization and dynamics of Cdc2-cyclin B during meiotic reinitiation in starfish oocytes. Mol Biol Cell 14:4685-94
Campagnola, Paul J; Millard, Andrew C; Terasaki, Mark et al. (2002) Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues. Biophys J 82:493-508
Hinkle, Beth; Slepchenko, Boris; Rolls, Melissa M et al. (2002) Chromosomal association of Ran during meiotic and mitotic divisions. J Cell Sci 115:4685-93

Showing the most recent 10 out of 12 publications