) This proposal addresses three important problem in biomedical science via the development of simple and rapid assays for biomolecules. The first goal involves the development of a room temperature color assay for fructose, a ubiquitous, rapidly metabolized sugar. The proposedassay is designed to be fructose-selective in the presence of a large excess of glucose. Fructose determination in plasma is currently hampered due to the large excess of glucose. The second goal involves creatinga mild assay for sialic acid, an important cell surface antigen, to address the problem of current assay inaccuracy based on hydrolytic procedures and/or relatively non-specific and harsh methods. The third goal entails the implementation of a new automated color detection system for oligosaccharides that does not require prior destructive hydrolysis or chemical labeling with a chromophore. Currently no detection technique exists for neutral oligosaccharides that is effective for the visual detection of oligosaccharides beyond three repeat units.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM061915-02
Application #
6520327
Study Section
Medicinal Chemistry Study Section (MCHA)
Program Officer
Edmonds, Charles G
Project Start
2001-05-01
Project End
2006-04-30
Budget Start
2002-05-01
Budget End
2003-04-30
Support Year
2
Fiscal Year
2002
Total Cost
$183,750
Indirect Cost
Name
Louisiana State University A&M Col Baton Rouge
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
075050765
City
Baton Rouge
State
LA
Country
United States
Zip Code
70803
Swanson, Douglas J; Steshina, Ekaterina Y; Wakenight, Paul et al. (2010) Phenotypic and genetic analysis of the cerebellar mutant tmgc26, a new ENU-induced ROR-alpha allele. Eur J Neurosci 32:707-16
He, Ming; Johnson, Rolanda J; Escobedo, Jorge O et al. (2002) Chromophore formation in resorcinarene solutions and the visual detection of mono- and oligosaccharides. J Am Chem Soc 124:5000-9