Abstract

We propose label-free high-throughput detection of cells response to chemicals by merging microwell microtechnology with state-of-the-art label-free biosensing technology based on optical microresonators. Arrays of optical microbead sensors will be sealed against arrays of cells in microwells to monitor secretion of protein in response to chemicals released from a hydrogel. The platform can be operated on a conventional total-internal-reflection microscope setup.

Public Health Relevance

Cells in microwell arrays fabricated from polyethyleneglycol polymer will be sealed against an array of optical microbead biosensors co-localized with a hydrogel array for controlled release of chemicals. Secreted apoptotic biomarkers such as caspase-3 protein will be monitored in label-free manner and in real-time in response to toxic chemicals released from the hydrogels.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM095906-02
Application #
8334471
Study Section
Instrumentation and Systems Development Study Section (ISD)
Program Officer
Edmonds, Charles G
Project Start
2011-09-19
Project End
2016-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
2
Fiscal Year
2012
Total Cost
$350,712
Indirect Cost
$100,712

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Wu, Fengchi; Wu, Yuqiang; Niu, Zhongwei et al. (2016) Integrating a DNA Strand Displacement Reaction with a Whispering Gallery Mode Sensor for Label-Free Mercury (II) Ion Detection. Sensors (Basel) 16:
Chen, Ying-Jen; Xiang, Wei; Klucken, Jochen et al. (2016) Tracking micro-optical resonances for identifying and sensing novel procaspase-3 protein marker released from cell cultures in response to toxins. Nanotechnology 27:164001
Wu, F C; Wu, Y; Niu, Z et al. (2015) Ratiometric detection of oligonucleotide stoichiometry on multifunctional gold nanoparticles by whispering gallery mode biosensing. Analyst 140:2969-72
Foreman, Matthew R; Swaim, Jon D; Vollmer, Frank (2015) Whispering gallery mode sensors. Adv Opt Photonics 7:168-240
Wilson, Kerry A; Finch, Craig A; Anderson, Phillip et al. (2015) Combining an optical resonance biosensor with enzyme activity kinetics to understand protein adsorption and denaturation. Biomaterials 38:86-96
Bertassoni, Luiz E; Cardoso, Juliana C; Manoharan, Vijayan et al. (2014) Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels. Biofabrication 6:024105
Bertassoni, Luiz E; Cecconi, Martina; Manoharan, Vijayan et al. (2014) Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. Lab Chip 14:2202-11
Shevchenko, Yanina; Camci-Unal, Gulden; Cuttica, Davide F et al. (2014) Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior. Biosens Bioelectron 56:359-67
Wu, Yuqiang; Zhang, David Yu; Yin, Peng et al. (2014) Ultraspecific and highly sensitive nucleic acid detection by integrating a DNA catalytic network with a label-free microcavity. Small 10:2067-76
Oh, Jonghyun; Kim, Keekyoung; Won, Sung Wook et al. (2013) Microfluidic fabrication of cell adhesive chitosan microtubes. Biomed Microdevices 15:465-72

Showing the most recent 10 out of 12 publications