The Mechanism of TTQ Biogenesis Project Description Understanding how an enzyme specifically oxidizes a protein substrate that is much larger than the enzyme itself will illuminate the mechanism by which proteins mature through enzyme-mediated posttranslational modifications. Given the interconnectedness of protein posttranslational modification, metabolic chemistry, and diseases, the question of how enzymes preserve specificity for large protein substrates is fundamental to enzymology. We are studying the long-range remote enzyme catalysis mechanism required for the biogenesis of a protein-derived tryptophan tryptophylquinone (TTQ) cofactor. TTQ is the catalytic center of methylamine dehydrogenase (MADH). This proposal seeks to determine the chemical properties of two reactive intermediates, an unprecedented bis-FeIV state of a di-heme enzyme MauG and a novel tryptophan-based di-radical in the substrate protein preMADH. Both are critical catalytic intermediates that occur sequentially in the catalytic cycle of TTQ biogenesis. Characterization of these key intermediates will lead to comprehension of the TTQ biogenesis mechanism, which in turn will provide insight into long-range enzyme-mediated remote oxidative and oxygenative chemical modification strategies. These studies will begin with examining the nature of the electronic interactions between the two hemes and the chemical reactivity, stability, and spectroscopic signature of the high-valent bis-FeIV state of MauG. Then, we will follow up with spectroscopic, structural, and theoretical characterizations of the tryptophan-based di-radical intermediate in preMADH to elucidate the coupling nature of the di- radical species and the proton release mechanism necessary for cross-linking and subsequent oxidation reactions.
Free radicals and reactive oxygen species are double-edged swords. On one hand, they are the primary culprits for non-specific oxidative damage to cell components, leading to oxidative stress, aging, and mitochondrial disease states. On the other hand, many critical metabolic events depend on protein-based free radicals and reactive oxygen species that are produced and consumed in a controlled manner. The proposed research will elucidate how an iron-dependent enzyme generates protein-based radicals in a tightly controlled manner to produce cofactors necessary for sustaining life.
Showing the most recent 10 out of 22 publications
