Sepsis and trauma are two of the most catastrophic challenges, and result in the rapid and immediate reprioritization of host protective immunity. We have used terms like ?genomic storm? and ?cytokine storm? to summarize the host immunological changes that accompany tissue damage or microbial infection. Controversially, we have argued that both trauma and sepsis are diseases of the bone marrow, and that ?emergency myelopoiesis? defines the bone marrow and secondary hematologic tissue responses to trauma and sepsis. We have spent the past decade characterizing both the mature blood and tissue immunological response, as well as the hematopoietic response in bone marrow, and we were the first to describe the expansion of immature myeloid populations in sepsis. We have also demonstrated the importance of beta adrenergic stimulation on hemato- and myelopoiesis. NIGMS-funded investigators in our group have been exploring the effect of age (very young and very old) on the hematopoietic response. For the past decade, we have used a Becton-Dickinson LSRII flow cytometer to phenotype human blood and bone marrow leukocytes, and murine blood and tissues following severe trauma and sepsis. Because human trauma and sepsis occur 24/7 (especially late at night and on weekends), the instrument is being used at all times of night and day, and is often required on short to immediate notice for samples from human trauma and sepsis. This precludes sharing an instrument with other entities. We have been informed that the current LSRII is at the end of its scheduled life-span and Becton-Dickinson will no longer service the instrument or provide replacement parts. We are therefore requesting funds to partially offset the cost of a new LSR-Fortessa, four-laser, 16 color flow cytometer as a shared instrument among five NIGMS-funded investigators. The department of surgery will contribute up to $80,000 towards its purchase. The instrument will replace the LSRII and will be available to all current LSRII users. The new instrument will provide additional capabilities which will increase our capacity to more precisely phenotype leukocyte populations, going to 16 analytes (colors) versus the current eight, making it possible to more accurately identify our leukocyte populations. In addition to its ability to better characterize the immature myeloid and hematopoietic stem cell populations, its sampling speed and additional colors will reduce significantly the labor involved in sample preparation and analysis. The LSRII has served us well for over a decade, but the new instrument is essential to maintain existing productivity and to improve the analytical capabilities of multiple NIGMS awardees.
Sepsis and trauma are immunological diseases, and phenotyping both blood and bone marrow leukocyte populations are essential to understanding the host protective response. This proposal requests partial support for the purchase of a four laser, 16 color LSR-Fortessa flow cytometer to replace an outdated and no longer supported LSRII flow cytometer currently used by five NIGMS funded investigators.
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| Loftus, Tyler J; Rosenthal, Martin D; Croft, Chasen A et al. (2018) The effects of beta blockade and clonidine on persistent injury-associated anemia. J Surg Res 230:175-180 |
| Horiguchi, Hiroyuki; Loftus, Tyler J; Hawkins, Russell B et al. (2018) Innate Immunity in the Persistent Inflammation, Immunosuppression, and Catabolism Syndrome and Its Implications for Therapy. Front Immunol 9:595 |
| Loftus, Tyler J; Brakenridge, Scott C; Croft, Chasen A et al. (2018) Successful nonoperative management of uncomplicated appendicitis: predictors and outcomes. J Surg Res 222:212-218.e2 |
| Mira, Juan C; Nacionales, Dina C; Loftus, Tyler J et al. (2018) Mouse Injury Model of Polytrauma and Shock. Methods Mol Biol 1717:1-15 |
| Loftus, Tyler J; Brakenridge, Scott C; Murphy, Travis W et al. (2018) Anemia and blood transfusion in elderly trauma patients. J Surg Res 229:288-293 |
| Loftus, Tyler J; Mira, Juan C; Stortz, Julie A et al. (2018) Persistent Inflammation and Anemia among Critically Ill Septic Patients. J Trauma Acute Care Surg : |
| Efron, Philip A; Mohr, Alicia M; Bihorac, Azra et al. (2018) Persistent inflammation, immunosuppression, and catabolism and the development of chronic critical illness after surgery. Surgery 164:178-184 |
| Loftus, Tyler J; Kannan, Kolenkode B; Carter, Christy S et al. (2018) Persistent injury-associated anemia in aged rats. Exp Gerontol 103:63-68 |
| Stortz, Julie A; Mira, Juan C; Raymond, Steven L et al. (2018) Benchmarking clinical outcomes and the immunocatabolic phenotype of chronic critical illness after sepsis in surgical intensive care unit patients. J Trauma Acute Care Surg 84:342-349 |
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