for Parent Grant: R01GM120349 This research will develop methods to model active sites in metalloproteins for the purpose of determining fundamental structure-function relationships for how proteins activate dioxygen, a process that strongly impacts human health and aging. Artificial metallproteins will be prepared utilizing biotin-streptavidin technology as a tool to ensure specific and reproducible placement of synthetic metal complexes within protein hosts. This approach is proposed to be an effective method to model key properties of the active sites in native metalloproteins, including site isolation of species, regulation of the primary coordination sphere, and control of the microenvironments around the metal complexes. One glaring weakness of many biomimetic systems is their limited ability to regulate the microenvironments that surround metal centers. No chemical system operates in isolation without interacting with its local environment. There is a growing body of evidence from structural biology that the microenvironment, a space around metal complexes that comprises the secondary coordination sphere, has profound effects on protein function that ranges from modulation of physical properties to delivery of reactants and removal of products. It is our contention that the greater regulation of microenvironments will lead to better understanding of protein function. It is further maintained that the benefits gained from fundamental analyses as proposed in this application extend well beyond improvements in selectivities/efficiencies at the molecular level ? they are transformative for all types of platforms, providing the requisite information that is still missing for the development of highly functional systems. We propose an approach for preparing artificial metalloproteins that allows for the confinement of synthetic complexes within protein hosts to regulate both the primary and secondary coordination spheres about the immobilized metal centers. The ability to regulate these coordination spheres within a protein will produce systematic structure-function relationships that will lead to an improved understanding of chemical processes that are directly linked to human health. 1
for Parent Grant: R01GM120349 Activation of small molecules (e.g, O2) by metalloproteins is a process that is directly linked to the maintenance of human health. Protein active sites are instrumental in regulating these essential processes. Thus the function, and dysfunction of key health related processes can be understood within the context of protein active site structures. Developing new methods to probe key structural aspects that control function is needed to expand on the potent functions of proteins in artificial systems. One approach, used in this application, is to prepare artificial metalloproteins that leverage the power of synthetic chemistry with molecular biology. This integrative approach provides a means to establish key structure-function relationships that are inherent for superior function.
| Olshansky, Lisa; Huerta-Lavorie, Raúl; Nguyen, Andy I et al. (2018) Artificial Metalloproteins Containing Co4O4 Cubane Active Sites. J Am Chem Soc 140:2739-2742 |
| Mann, Samuel I; Heinisch, Tillmann; Ward, Thomas R et al. (2018) Coordination chemistry within a protein host: regulation of the secondary coordination sphere. Chem Commun (Camb) 54:4413-4416 |
| Mann, Samuel I; Heinisch, Tillmann; Ward, Thomas R et al. (2017) Peroxide Activation Regulated by Hydrogen Bonds within Artificial Cu Proteins. J Am Chem Soc 139:17289-17292 |
| Mann, Samuel I; Heinisch, Tillmann; Weitz, Andrew C et al. (2016) Modular Artificial Cupredoxins. J Am Chem Soc 138:9073-6 |
