Abstract

Gap junctions can now be characterized in terms of at least five parameters: conductance, permeability, pH dependence, divalent ion dependence, and voltage dependence. We are analyzing these properties in terms of interactions between treatments and effects of other treatments such as octanol, aldehyde fixatives and group-specific protein reagents. Physiological studies are correlated with fine structural investigations involving freeze fracture and thin sectioning. The goals are to evaluate single channel conductance and permeability and physiological factors controlling conductance. We hope to implicate specific amino acid residues as involved in gating and integrity of junctional membranes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD004248-17
Application #
3310248
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1978-09-29
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
17
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
Schools of Medicine
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Anand, Shikha G; Mehta, Supriya D; Adams, William G (2006) Diabetes mellitus screening in pediatric primary care. Pediatrics 118:1888-95
Bennett, M V; Zheng, X; Sogin, M L (1994) The connexins and their family tree. Soc Gen Physiol Ser 49:223-33
Zheng, X; Zhang, L; Durand, G M et al. (1994) Mutagenesis rescues spermine and Zn2+ potentiation of recombinant NMDA receptors. Neuron 12:811-8
Durand, G M; Bennett, M V; Zukin, R S (1993) Splice variants of the N-methyl-D-aspartate receptor NR1 identify domains involved in regulation by polyamines and protein kinase C. Proc Natl Acad Sci U S A 90:6731-5
Bennett, M V; Rubin, J B; Bargiello, T A et al. (1993) Structure-function studies of voltage sensitivity of connexins, the family of gap junction forming proteins. Jpn J Physiol 43 Suppl 1:S301-10
Araneda, R C; Zukin, R S; Bennett, M V (1993) Effects of polyamines on NMDA-induced currents in rat hippocampal neurons: a whole-cell and single-channel study. Neurosci Lett 152:107-12
Durand, G M; Gregor, P; Zheng, X et al. (1992) Cloning of an apparent splice variant of the rat N-methyl-D-aspartate receptor NMDAR1 with altered sensitivity to polyamines and activators of protein kinase C. Proc Natl Acad Sci U S A 89:9359-63
Rubin, J B; Verselis, V K; Bennett, M V et al. (1992) Molecular analysis of voltage dependence of heterotypic gap junctions formed by connexins 26 and 32. Biophys J 62:183-93;discussion 193-5
Rubin, J B; Verselis, V K; Bennett, M V et al. (1992) A domain substitution procedure and its use to analyze voltage dependence of homotypic gap junctions formed by connexins 26 and 32. Proc Natl Acad Sci U S A 89:3820-4
Bennett, M V; Verselis, V K (1992) Biophysics of gap junctions. Semin Cell Biol 3:29-47

Showing the most recent 10 out of 22 publications