Gonadotropins from the horse and related species are employed as models because of their unique properties (high carbohydrate content; dual biological activities) to obtain information on the chemical, immunological and biological properties of gonadotropins which will relate to their mechanism of action. Techniques of protein chemistry are used to isolate and characterize highly purified equine and related species of luteinizing hormone, follicle stimulating hormone and chorionic gonadotropin. In vitro bioassays, radioreceptor assays and radioimmunoassays are used to obtain structure-function information.
The specific aims of the grant proposal are to extensively compare the properties of biosynthetic equine gonadotropins prepared by recombinant DNA technology, to study the role of gonadotropin carbohydrate with intact oligosaccharide units devoid of peptide and by deglycosylation reactions, to isolate and characterize isohormonal forms of equine pituitary gonadotropins and to compare them with secreted forms, to study the gonadotropins in a closely related equid species, the zebra, and to study the activities of equine gonadotropin hybrid molecules prepared from the subunits of molecules with varying activities and potencies. These studies are intended to increase our knowledge of the mechanism of gonadotropin action and reproductive function and should be of future value in devising new approaches to the regulation and maintenance of human reproduction.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD005722-17
Application #
3310410
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1978-07-01
Project End
1991-06-30
Budget Start
1989-07-01
Budget End
1990-06-30
Support Year
17
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
McFarlane, J R; Cabrera, C M; Coulson, S A et al. (1991) Partial purification and characterization of rhinoceros gonadotropins, growth hormone, and prolactin: comparison with the horse and sheep. Biol Reprod 44:94-101
McFarlane, J R; Czekala, N M; Papkoff, H (1991) Zebra chorionic gonadotropin: partial purification and characterization. Biol Reprod 44:827-33
McFarlane, J R; Coulson, S A; Papkoff, H (1991) Biological and immunoactive substances resembling chorionic gonadotropin are present in full-term horse and zebra placentas. Biol Reprod 45:343-9
Tortonese, D J; Lewis, P E; Papkoff, H et al. (1990) Roles of the dominant follicle and the pattern of oestradiol in induction of preovulatory surges of LH and FSH in prepubertal heifers by pulsatile low doses of LH. J Reprod Fertil 90:127-35
McFarlane, J R; Cabrera, C M; Oosthuizen, M M et al. (1990) Elephant pituitary gonadotropins. Gen Comp Endocrinol 79:193-200
Bergert, E R; Madden, B; McCormick, D J et al. (1990) The antigenic structure of the human glycoprotein hormone alpha-subunit: II. Cross-species comparisons. Endocrinology 127:2985-9
Matteri, R L; Papkoff, H (1988) Microheterogeneity of equine follicle-stimulating hormone. Biol Reprod 38:324-31
Matteri, R L; Papkoff, H (1987) Characterization of equine luteinizing hormone by chromatofocusing. Biol Reprod 36:261-9
Matteri, R L; Baldwin, D M; Lasley, B L et al. (1987) Biological and immunological properties of zebra pituitary gonadotropins: comparison with horse and donkey gonadotropins. Biol Reprod 36:1134-41
Matteri, R L; Roser, J F; Baldwin, D M et al. (1987) Characterization of a monoclonal antibody which detects luteinizing hormone from diverse mammalian species. Domest Anim Endocrinol 4:157-65

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