Abstract

The basic aim of the proposed research is to characterize stage-specific surface proteins (SSSP) and to examine their role during early development and differentiation of mouse embryo. Syngeneic immunization with embryos of different developmental stages will be used to obtain specific antisera. Surface proteins of preimplantation mouse embryos will be labeled, immunoprecipitated with specific antisera and characterized on one- and two-dimensional gels. Spleen cells from immunized mice will be fused with myeloma cells and hybrids producing monoclonal antibodies against stage-specific surface proteins isolated. Teratocarcinoma stem cell lines will be tested with anti-embryo sera and those with cross-reacting antigens detected. Using monoclonal antibodies and teratocarcinoma stem cells, a sufficient amount of SSSP will be isolated and characterized in detail. Anti-embryo sera will be used to probe the properties of the embryonic cell membrane and to assess the role of SSSP in differentiation and development of embryos and teratocarcinoma stem cells in vitro.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD012487-06
Application #
3311891
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1979-03-01
Project End
1987-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Wistar Institute
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Solter, D (1988) Differential imprinting and expression of maternal and paternal genomes. Annu Rev Genet 22:127-46
Aronson, J; Solter, D (1987) Developmental potency of gametic and embryonic genomes revealed by nuclear transfer. Curr Top Dev Biol 23:55-71
Fox, N; Solter, D (1987) Expression and cAMP-mediated regulation of the human gonadotropin alpha subunit gene in transfected mouse L-cells. Differentiation 36:255-9
Liebert, M; Jaffe, R; Taylor, R J et al. (1987) Detection of SSEA-1 on human renal tumors. Cancer 59:1404-8
Ballou, B; Reiland, J M; Levine, G et al. (1987) Radioimmunolocation of a heterotransplanted human choriocarcinoma (BeWo) using monoclonal anti-SSEA-1: pharmacokinetics. Hybridoma 6:241-51
McGrath, J; Solter, D (1986) Nucleocytoplasmic interactions in the mouse embryo. J Embryol Exp Morphol 97 Suppl:277-89
Tippett, P; Andrews, P W; Knowles, B B et al. (1986) Red cell antigens P (globoside) and Luke: identification by monoclonal antibodies defining the murine stage-specific embryonic antigens -3 and -4 (SSEA-3 and SSEA-4). Vox Sang 51:53-6
Howe, C C; Overton, G C (1986) Expression of the intracisternal A-particle is elevated during differentiation of embryonal carcinoma cells. Mol Cell Biol 6:150-7
McGrath, J; Solter, D (1986) Nuclear and cytoplasmic transfer in mammalian embryos. Dev Biol (N Y 1985) 4:37-55
Ballou, B; Reiland, J M; Levine, G et al. (1986) Tumor location and drug targeting using a monoclonal antibody (anti-SSEA-1) and antigen-binding fragments. J Surg Oncol 31:1-12

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