Abstract

This application proposes to identify which synapses are involved in phased synaptic remodeling. By quantitative ultra-structural morphometry and synapse counts, the effects of ovariectomy (low estrogen), progesterone, and ring A-reduced androgens will be studied. Freeze-fracture analysis will be used to determine whether intramembranous protein particle(IMP) deletion regularly precedes synaptic changes and whether there is a critical level of IMP density in postsynaptic membranes below which it is not possible to maintain cyclic circuitry. Neuronal proliferation and differentiation in fetal hypothalamic cell cultures will be studied utilizing cell- and molecular biology methods to probe the appearance of oncogenes and intracellular/membrane proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD013587-11
Application #
3312246
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1980-09-30
Project End
1995-07-31
Budget Start
1991-08-01
Budget End
1992-07-31
Support Year
11
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Naftolin, Frederick; Malaspina, Dolores (2007) Estrogen, estrogen treatment and the post-reproductive woman's brain. Maturitas 57:23-6
Naftolin, F; Horvath, T L; Balthazart, J (2001) Estrogen synthetase (aromatase) immunohistochemistry reveals concordance between avian and rodent limbic systems and hypothalami. Exp Biol Med (Maywood) 226:717-25
Keefe, D L; Watson, R; Naftolin, F (1999) Hormone replacement therapy may alleviate sleep apnea in menopausal women: a pilot study. Menopause 6:196-200
Diano, S; Naftolin, F; Horvath, T L (1997) Gonadal steroids target AMPA glutamate receptor-containing neurons in the rat hypothalamus, septum and amygdala: a morphological and biochemical study. Endocrinology 138:778-89
Horvath, T L; Roa-Pena, L; Jakab, R L et al. (1997) Aromatase in axonal processes of early postnatal hypothalamic and limbic areas including the cingulate cortex. J Steroid Biochem Mol Biol 61:349-57
Fernandez-Galaz, M C; Morschl, E; Chowen, J A et al. (1997) Role of astroglia and insulin-like growth factor-I in gonadal hormone-dependent synaptic plasticity. Brain Res Bull 44:525-31
Horvath, T L; Garcia-Segura, L M; Naftolin, F (1997) Lack of gonadotropin-positive feedback in the male rat is associated with lack of estrogen-induced synaptic plasticity in the arcuate nucleus. Neuroendocrinology 65:136-40
Parducz, A; Szilagyi, T; Hoyk, S et al. (1996) Neuroplastic changes in the hypothalamic arcuate nucleus: the estradiol effect is accompanied by increased exoendocytotic activity of neuronal membranes. Cell Mol Neurobiol 16:259-69
Garcia-Segura, L M; Chowen, J A; Duenas, M et al. (1996) Gonadal steroids and astroglial plasticity. Cell Mol Neurobiol 16:225-37
Duenas, M; Torres-Aleman, I; Naftolin, F et al. (1996) Interaction of insulin-like growth factor-I and estradiol signaling pathways on hypothalamic neuronal differentiation. Neuroscience 74:531-9

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