To investigate cell surface changes associated with the development of uterine receptivity for ovoimplantation, isolated rabbit uterine epithelial cells will be maintained in primary cell culture. The cells will be incubated with [32P] orthophosphate and [3H] glucosamine, and patterns of labelled proteins in purified plasma membranes will be examined by polyacrylamide gel electrophoresis. Monoclonal antibodies prepared against epithelial plasma membrances will be screened against primary cultures of receptive and nonreceptive epithelial cells to detect stage-specific surface antigens. A functional role for such antigens in cell adhesion will be tested by use of stagespecific antibodies to block the binding of 125I-trophoblast membranes to the surface of epithelial cells in culture, and to epithelial plasma membrane proteins separated by electrophoresis and transferred to nitrocellulose filters. Failure of implantation is a common cause of human infertility. The results will provide evidence for the molecular basis of trophoblast-uterine epithelial cell attachment at ovoimplantation in an animal model.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD013663-06
Application #
3312263
Study Section
Reproductive Biology Study Section (REB)
Project Start
1980-04-01
Project End
1986-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
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Mani, S K; Carson, D D; Glasser, S R (1992) Steroid hormones differentially modulate glycoconjugate synthesis and vectorial secretion by polarized uterine epithelial cells in vitro. Endocrinology 130:240-8
Mani, S K; Decker, G L; Glasser, S R (1991) Hormonal responsiveness by immature rabbit uterine epithelial cells polarized in vitro. Endocrinology 128:1563-73
Ricketts, A P; Scott, D W; Bullock, D W (1986) Radioiodinated surface proteins of rabbit trophectoderm cells. Cell Tissue Res 246:667-71
Lampelo, S A; Anderson, T L; Bullock, D W (1986) Monoclonal antibodies recognize a cell surface marker of epithelial differentiation in the rabbit reproductive tract. J Reprod Fertil 78:663-72
Lampelo, S A; Ricketts, A P; Bullock, D W (1985) Purification of rabbit endometrial plasma membranes from receptive and non-receptive uteri. J Reprod Fertil 75:475-84