How a multipotent cell becomes committed to a particular differentiation pathway is not known. The long term goal of this research is to understand the controls governing the proliferation and differentiation behavior of a multipotent stem cell, the interstitial cell of hydra. At this stage, the approach is to elucidate the cell-cell interactions regulating each of the several differentiations the interstitial cell can undergo. It is known that the interstitial cell population contains multipotent stem cells and cells committed to a particular differentiation. There is increasing evidence that for this system, and every other system with a multipotent cell, there are cells with intermediate differentiation capacities. Thus, any cell-cell interaction, or cell-molecule interaction thought to affect the multipotent cell, may yield ambiguous results as it may affect a derivative cell instead. Therefore to accurately work out the controls effecting the multipotent cell, a precise knowledge of the complexity of the population in terms of number of cell types with different differentiation capabilities and of the cell lineage relationships among them is necessary. The goals of this proposal are to do that for the interstitial cell system of hydra with the following approach: (1) Monoclonal antibodies that bind specifically to I-cells, to subsets of I-cells, to subsets of I-cells and differentiation intermediates and/or products will be sought. (2) Subsets of I-cells, each defined by a monoclonal antibody, will be examined in pairs to determine their relatedness. This will lead to a map of nearest neighbors which will tentatively be a map of the cell lineage relationships. (3) Using the defining antibody, cells of each subset will be isolated and their differentiation and proliferation capacities examined. This information will be used to validate the relationships described in the map. An understanding of the cellular interactions controlling the differentiation of multipotent cells in basic to an understanding of some classes of defects that occur during human development.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD016440-05
Application #
3313684
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1982-04-01
Project End
1988-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
5
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of California Irvine
Department
Type
Schools of Arts and Sciences
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697
Koizumi, O; Bode, H R (1991) Plasticity in the nervous system of adult hydra. III. Conversion of neurons to expression of a vasopressin-like immunoreactivity depends on axial location. J Neurosci 11:2011-20
Bode, H R; Gee, L W; Chow, M A (1990) Neuron differentiation in hydra involves dividing intermediates. Dev Biol 139:231-43
Koizumi, O; Heimfeld, S; Bode, H R (1988) Plasticity in the nervous system of adult hydra. II. Conversion of ganglion cells of the body column into epidermal sensory cells of the hypostome. Dev Biol 129:358-71
Javois, L C; Wood, R D; Bode, H R (1986) Patterning of the head in hydra as visualized by a monoclonal antibody. I. Budding and regeneration. Dev Biol 117:607-18
Yu, S M; Westfall, J A; Dunne, J F (1986) Use of a monoclonal antibody to classify neurons isolated from the head region of Hydra. J Morphol 188:79-90
Javois, L C; Iten, L E (1986) The handedness and origin of supernumerary limb structures following 180 degrees rotation of the chick wing bud on its stump. J Embryol Exp Morphol 91:135-52
Koizumi, O; Bode, H R (1986) Plasticity in the nervous system of adult hydra. I. The position-dependent expression of FMRFamide-like immunoreactivity. Dev Biol 116:407-21
Bode, H; Dunne, J; Heimfeld, S et al. (1986) Transdifferentiation occurs continuously in adult hydra. Curr Top Dev Biol 20:257-80
Littlefield, C L (1986) Sex determination in hydra: control by a subpopulation of interstitial cells in Hydra oligactis males. Dev Biol 117:428-34
Yaross, M S (1986) Analysis of hydra interstitial cell potential using viable cell sorting. Prog Clin Biol Res 217B:51-4

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