Abstract

The respective roles of nucleus and cityoplasm in early mammalian development are little understood. What effect maternally inherited cytoplasmic components have on differentiation, and the developmental stage to which they persist are largely unknown. We propose to investigate nuclear/cytoplasmic interactions in the mouse embryo using a novel nuclear tranplantation technique. This technique will be employed to determine the ability of nuclear transplant empryos to undergo normal development and also define the developmental stage at which nuclear endoced gene products can first be detected. Possible maternally derived cytoplasmic contributions to the embryonic phenotype will also be investigated. Experiments regarding the latter were specifically chosen to define possible cytoplasmic effects at different stages of development (ranging from early preimplantation development to late gestational embryogenesis). It is hoped that these experiments will delineate the nuclear/cytoplasmic origin of embryonic gene products and events in addition to increasing our understanding of the biology of nuclear transplantation in mammals.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD017720-03
Application #
3314736
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1983-08-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Wistar Institute
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Latham, K E; Garrels, J I; Solter, D (1993) Two-dimensional gel analysis of protein synthesis. Methods Enzymol 225:473-89
Latham, K E; Solter, D (1993) Transplantation of nuclei to oocytes and embryos. Methods Enzymol 225:719-32
Latham, K E; Beddington, R S; Solter, D et al. (1993) Quantitative analysis of protein synthesis in mouse embryos. II: Differentiation of endoderm, mesoderm, and ectoderm. Mol Reprod Dev 35:140-50
Lamb, B T; Satyamoorthy, K; Solter, D et al. (1992) A DNA element that regulates expression of an endogenous retrovirus during F9 cell differentiation is E1A dependent. Mol Cell Biol 12:4824-33
Latham, K E; Solter, D; Schultz, R M (1992) Acquisition of a transcriptionally permissive state during the 1-cell stage of mouse embryogenesis. Dev Biol 149:457-62
Latham, K E; Garrels, J I; Chang, C et al. (1992) Analysis of embryonic mouse development: construction of a high-resolution, two-dimensional gel protein database. Appl Theor Electrophor 2:163-70
Latham, K E; Solter, D (1991) Effect of egg composition on the developmental capacity of androgenetic mouse embryos. Development 113:561-8
Lamb, B T; Satyamoorthy, K; Li, L et al. (1991) CpG methylation of an endogenous retroviral enhancer inhibits transcription factor binding and activity. Gene Expr 1:185-96
Latham, K E; Solter, D; Schultz, R M (1991) Activation of a two-cell stage-specific gene following transfer of heterologous nuclei into enucleated mouse embryos. Mol Reprod Dev 30:182-6
Latham, K E; Garrels, J I; Chang, C et al. (1991) Quantitative analysis of protein synthesis in mouse embryos. I. Extensive reprogramming at the one- and two-cell stages. Development 112:921-32

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