Abstract

The overall aim of this work is to understand the biochemical effects of androgens or testicular cells and to relate these to the effects of FSH and to the control of the spermatogenic process. The approach will involve delineation of similarities and differences between the protein synthetic response of Sertoli and peritubular cells using 35S-Met- labeling and 2D-PAGE. The cellular localization and regulation of testicular gamma-glutamyl transpeptidase mRNA will be studied with the use of cDNA probes. cDNA probes for hormonally regulated Sertoli cell mRNA will be isolated using 32p-Sertoli cell cDNA enriched for hormone- dependent messages by subtractive prehybridization with respect to sequence and subcloned into open reading frame vectors and the resulting tribrid proteins used to prepare antibodies. The antibodies in turn will be used to determine the subcellular localization and possible identity of the corresponding protein. These studies should increase our understanding of the hormonal control of Sertoli cell functions and provide new insight into aspects of the functions which may relate to the control of spermatogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
2R01HD017795-05A2
Application #
3314808
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1983-07-01
Project End
1991-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center Houston
Department
Type
Schools of Medicine
DUNS #
City
Houston
State
TX
Country
United States
Zip Code
77225

  Publications

Lu, Q; Porter, L D; Cui, X et al. (2001) Ecto-ATPase mRNA is regulated by FSH in Sertoli cells. J Androl 22:289-301
Walker, W H; Sanborn, B M; Habener, J F (1994) An isoform of transcription factor CREM expressed during spermatogenesis lacks the phosphorylation domain and represses cAMP-induced transcription. Proc Natl Acad Sci U S A 91:12423-7
Ku, C Y; Loose-Mitchell, D S; Sanborn, B M (1994) Both Sertoli and peritubular cells respond to androgens with increased expression of an androgen response element reporter. Biol Reprod 51:319-26
Jakubowiak, A; Janecki, A; Tong, D et al. (1991) Effects of recombinant human inhibin and testosterone on gonadotropin secretion and subunit mRNA in superfused male rat pituitary cell cultures stimulated with pulsatile gonadotropin-releasing hormone. Mol Cell Endocrinol 82:265-73
Ku, C Y; Lu, Q; Ussuf, K K et al. (1991) Hormonal regulation of cytochrome oxidase subunit messenger RNAs in rat Sertoli cells. Mol Endocrinol 5:1669-76
Sanborn, B M; Caston, L A; Chang, C et al. (1991) Regulation of androgen receptor mRNA in rat Sertoli and peritubular cells. Biol Reprod 45:634-41
Buzek, S W; Sanborn, B M (1990) Nuclear androgen receptor dynamics in testicular peritubular and Sertoli cells. J Androl 11:514-20
Caston, L A; Sanborn, B M (1988) Regulation of testicular and Sertoli cell gamma-glutamyl transpeptidase by follicle-stimulating hormone. Biol Reprod 38:109-13
Buzek, S W; Sanborn, B M (1988) Increase in testicular androgen receptor during sexual maturation in the rat. Biol Reprod 39:39-49
Sanborn, B M; Caston, L A; Buzek, S W et al. (1987) Hormonal regulation of Sertoli cell function. Adv Exp Med Biol 219:561-88

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