We will continue our study of specific aspects of prenatalpituituary gland development in the rat. Our goal is to determine the role that different tissue interactions have in establishing lobe-specific biochemical difference between anterior and intermediate lobes of the pituitary gland, which have a common embryological origin (Rathke's pouch). We will continue to study differentiation of proopiomelancortin (POMC)-containing cells that are present in both anterior and intermediate lobes the pituitary but which differentiate at different times during development, process POMC to different end product hormone, and are differentially regulated in the adult. 1. We will continue to study aspects of fetal POMC processing. We will analyze processing of POMC-oligosaccharides in order to determine the intracellular compartment at which specific processing events occur during development and also examine the appearance of an adult-intermediate lobe specific processing gene by Northern blot analysis and in situ hybridization. 2. We will use the reverse-hemolytic plaque assay to examine the development of basal and stimulated secretory potential by POMC cells in both lobes and complement this analysis by using cDNA probes to the glucocoticoid receptor for in situ hybridization and Northern blot analysis. 3. We will examine the differentiative capacity of intermediate lobe POMC-producing cells in the presence or absence of infundibulum in vitro. 4. Finally, we will make and characterize antibodies to monoamine oxidase-A to complement our analysis of MAO-B and thus to provide insight into the regulation of prenatal pituitary function by MAO substrates such as serotonin noradrenaline, and dopamine.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD018592-06
Application #
3315678
Study Section
Human Embryology and Development Subcommittee 2 (HED)
Project Start
1986-08-01
Project End
1989-07-31
Budget Start
1988-08-01
Budget End
1989-07-31
Support Year
6
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Type
Schools of Medicine
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027
Zheng, M; Pintar, J E (1997) Ontogeny of prohormone convertases in rat prenatal development. Ann N Y Acad Sci 814:108-24
Lugo, D I; Pintar, J E (1996) Ontogeny of basal and regulated proopiomelanocortin-derived peptide secretion from fetal and neonatal pituitary intermediate lobe cells: melanotrophs exhibit transient glucocorticoid responses during development. Dev Biol 173:110-8
Lugo, D I; Pintar, J E (1996) Ontogeny of basal and regulated secretion from POMC cells of the developing anterior lobe of the rat pituitary gland. Dev Biol 173:95-109
Fliegner, K H; Kaplan, M P; Wood, T L et al. (1994) Expression of the gene for the neuronal intermediate filament protein alpha-internexin coincides with the onset of neuronal differentiation in the developing rat nervous system. J Comp Neurol 342:161-73
Zheng, M; Streck, R D; Scott, R E et al. (1994) The developmental expression in rat of proteases furin, PC1, PC2, and carboxypeptidase E: implications for early maturation of proteolytic processing capacity. J Neurosci 14:4656-73
Rogler, L E; Pintar, J E (1993) Expression of the P450 side-chain cleavage and adrenodoxin genes begins during early stages of adrenal cortex development. Mol Endocrinol 7:453-61
Scott, R E; Pintar, J E (1993) Developmental regulation of proopiomelanocortin gene expression in the fetal and neonatal rat pituitary. Mol Endocrinol 7:585-96
Kirchgessner, A L; Pintar, J E (1991) Guinea pig pancreatic ganglia: projections, transmitter content, and the type-specific localization of monoamine oxidase. J Comp Neurol 305:613-31
Gershon, M D; Sherman, D L; Pintar, J E (1990) Type-specific localization of monoamine oxidase in the enteric nervous system: relationship to 5-hydroxytryptamine, neuropeptides, and sympathetic nerves. J Comp Neurol 301:191-213
Scott, R E; Autelitano, D J; Lugo, D I et al. (1990) Developmental changes in levels of proopiomelanocortin intron A-containing heterogeneous nuclear RNA and mature messenger RNA in the anterior and neurointermediate lobes of the rat pituitary. Mol Endocrinol 4:812-20

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