Abstract

The investigators have previously demonstrated that neonatal animals and humans possess a neutrophil system which is small, easily exhausted by infection, and unable to re-expand quickly. The present studies are designed to establish a model of neutrophil production, migration and kinetics in the developing rat, and to develop new strategies for managing neutropenic septic neonates. One group of experiments will examine the development of mature neutrophil and macrophage pools, as well as their precursors, during fetal life. In another set of experiments, the kinetics of neutrophils during experimental neonatal meningitis will be examined, as will their response to various therapeutic maneuvers. Finally, cytoplasts will be tested as potential substitutes for neutrophil transfusion, and attempts will be made to improve their function by intracellular loading with molecules conjugated to polylysine. It is anticipated that these studies will further clarify the factors which regulate the development of fetal granulocytopoiesis, and provide direction for new clinical strategies to manage the consequences of inadequate neonatal neutrophil supply.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD022083-03
Application #
3321409
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1986-08-01
Project End
1990-07-31
Budget Start
1988-08-01
Budget End
1990-07-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Type
Schools of Medicine
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112