Many gonadal-steroid dependent neuroendocrine events and behaviors are thought to be mediated by steroid hormones acting through preoptic, hypothalamic and limbic system neurons which contain steroid-specific receptor proteins. The experiments in this proposal are designed to reveal, on a cell-by-cell basis (IA) the extrahypothalamic axonal connections of gonadal steroid hormone-concentrating neurons by using steroid autoradiography combined with retrograde neuroanatomical tracing. These experiments will reveal exactly how steroid concentrating neurons fit in neuroendocrinologically of behaviorally relevant neural circuits. Other experiments (IB) are designed to detect the chemical content, neurotransmitter or neuropeptide-related, of gonadal steroid hormone-concentrating neurons, also on a cell-by-cell basis, using steroid autoradioagraphy combined with immunocytochemistry. These neurons could be part of the mechanism by which gonadal steroid hormones influence neurotransmitter or neuropeptide regulation of hypothalamic releasing hormones, which subsequently effect anterior pituitary secretion. Experiments (II) designed to examine the neuroendocrine properties of intrinsic neurons of the POA or medial basal hypothalamus (MBH) will identify steroid-concentrating capacity, or neurotransmitter or neuropeptide content, of neurons that survive surgical isolation of islands of POA or MBH. The physiologic importance of the intrinsic hypothalamic neurons may be considerable; cell-by-cell analysis of their properties has yet to be carried out. The action of steroid hormones on the genome of cells that contain specific steroid hormone receptors is the best documented mechanism by which steroids influence cells. Experiments (III) designed to optimize the use of in situ hybridization with cDNA probes on cells of the brain will be carried out using probes to 1) prooxytocin; 2) provasopressin and 3) tyrosine hydroxylase. Then, these procedures will be used in a paradigm designed to demonstrate qualitatively and quantitatively the effects of physiological state or steroid hormones on mRNA levels of hypothalamic neurons, detected in situ with the cDNA probes. Limited attempts (IV) to analyze the estradiol receptor (E-R) content of hypothalamic neurons with immunocytochemical methods using primary antibodies to the E-R will be carried out. The long term goal of all these experiments is to reveal, on a cell-by-cell basis, the characteristics of gonadal.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD022983-03
Application #
3322979
Study Section
Endocrinology Study Section (END)
Project Start
1986-09-01
Project End
1990-05-31
Budget Start
1988-06-01
Budget End
1989-05-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Rutgers University
Department
Type
Graduate Schools
DUNS #
130029205
City
Newark
State
NJ
Country
United States
Zip Code
07102
Felton, T M; Linton, L; Rosenblatt, J S et al. (1999) First and second order maternal behavior related afferents of the lateral habenula. Neuroreport 10:883-7
Wagner, C K; Silverman, A J; Morrell, J I (1998) Evidence for estrogen receptor in cell nuclei and axon terminals within the lateral habenula of the rat: regulation during pregnancy. J Comp Neurol 392:330-42
Wagner, C K; Morrell, J I (1997) Neuroanatomical distribution of aromatase MRNA in the rat brain: indications of regional regulation. J Steroid Biochem Mol Biol 61:307-14
Bakowska, J C; Morrell, J I (1997) Atlas of the neurons that express mRNA for the long form of the prolactin receptor in the forebrain of the female rat. J Comp Neurol 386:161-77
Wagner, C K; Morrell, J I (1996) Levels of estrogen receptor immunoreactivity are altered in behaviorally-relevant brain regions in female rats during pregnancy. Brain Res Mol Brain Res 42:328-36
Wagner, C K; Morrell, J I (1996) Distribution and steroid hormone regulation of aromatase mRNA expression in the forebrain of adult male and female rats: a cellular-level analysis using in situ hybridization. J Comp Neurol 370:71-84
Wagner, C K; Morrell, J I (1995) In situ analysis of estrogen receptor mRNA expression in the brain of female rats during pregnancy. Brain Res Mol Brain Res 33:127-35
Hnatczuk, O C; Morrell, J I (1995) Interaction of male sensory cues and estradiol in the induction of estrus in the prairie vole. Physiol Behav 58:785-90
Corodimas, K P; Rosenblatt, J S; Matthews, T M et al. (1995) Neuroanatomical tract tracing provides histological verification of neuron loss following cytotoxic lesions. J Neurosci Methods 56:71-5
DonCarlos, L L; Malik, K; Morrell, J I (1995) Region-specific effects of ovarian hormones on estrogen receptor immunoreactivity. Neuroreport 6:2054-8

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